ArticleJournal of cellular and molecular medicine2020
A functional genomics approach to investigate the differentiation of iPSCs into lung epithelium at air-liquid interface.
Article in Journal of cellular and molecular medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 11 citations in OpenAlex.
- CFTR Gene Regulation in Human Pancreatic Duct, Bile Duct and Sweat Gland Epithelial Cells.Journal of cellular and molecular medicine · 2025Article
- Enhancement of airway epithelial cell differentiation by pulmonary endothelial cell co-culture.Stem cell research · 2022Article
- Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells.Tissue engineering and regenerative medicine · 2022Article
- Metabolomic profiling of human pluripotent stem cell differentiation into lung progenitors.iScience · 2022Article
- Article
- A new platform for high-throughput therapy testing on iPSC-derived lung progenitor cells from cystic fibrosis patients.Stem cell reports · 2021Article
- Somatic cell hemoglobin modulates nitrogen oxide metabolism in the human airway epithelium.Scientific reports · 2021Article
- OTX2 regulates CFTR expression during endoderm differentiation and occupies 3' cis-regulatory elements.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Article
- The Bromodomain Containing 8 (BRD8) transcriptional network in human lung epithelial cells.Molecular and cellular endocrinology · 2021Article
- A functional genomics approach to investigate the differentiation of iPSCs into lung epithelium at air-liquid interface.Journal of cellular and molecular medicine · 2020Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The availability of robust protocols to differentiate induced pluripotent stem cells (iPSCs) into many human cell lineages has transformed research into the origins of human disease. The efficacy of differentiating iPSCs into specific cellular models is influenced by many factors including both intrinsic and extrinsic features. Among the most challenging models is the generation of human bronchial epithelium at air-liquid interface (HBE-ALI), which is the gold standard for many studies of respiratory diseases including cystic fibrosis. Here, we perform open chromatin mapping by ATAC-seq and transcriptomics by RNA-seq in parallel, to define the functional genomics of key stages of the iPSC to HBE-ALI differentiation. Within open chromatin peaks, the overrepresented motifs include the architectural protein CTCF at all stages, while motifs for the FOXA pioneer and GATA factor families are seen more often at early stages, and those regulating key airway epithelial functions, such as EHF, are limited to later stages. The RNA-seq data illustrate dynamic pathways during the iPSC to HBE-ALI differentiation, and also the marked functional divergence of different iPSC lines at the ALI stages of differentiation. Moreover, a comparison of iPSC-derived and lung donor-derived HBE-ALI cultures reveals substantial differences between these models.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.