ArticleBiotechnology letters2020
Enhancement of glucaric acid production in Saccharomyces cerevisiae by expressing Vitreoscilla hemoglobin.
Article in Biotechnology letters, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- A functional and applied perspective of Vitreoscilla hemoglobin: from oxygen carriage to biotechnological innovation.Biotechnology letters · 2025Review
- 4-O-Methylglucaric Acid Production from Xylan with Uronic Acid Oxidase and Comparison to Glucaric Acid from Glucose.Chembiochem : a European journal of chemical biology · 2025Article
- Improving glucose oxidase catalysis in Aspergillus niger via Vitreoscilla hemoglobin fusion protein.Applied microbiology and biotechnology · 2024Article
- Cell factories for biosynthesis of D-glucaric acid: a fusion of static and dynamic strategies.World journal of microbiology & biotechnology · 2024Review
- Production of D-glucaric acid with phosphoglucose isomerase-deficient Saccharomyces cerevisiae.Biotechnology letters · 2024Article
- Efficient Production of Glucaric Acid by Engineered Saccharomyces cerevisiae.Applied and environmental microbiology · 2023Article
- Natural Clerodendrum-derived tick repellent: learning from Nepali culture.Experimental & applied acarology · 2023Article
- Implementation of Synthetic Pathways to Foster Microbe-Based Production of Non-Naturally Occurring Carboxylic Acids and Derivatives.Journal of fungi (Basel, Switzerland) · 2021Review
- Recent Advances in the Physicochemical Properties and Biotechnological Application ofMicroorganisms · 2021Review
- Exploration and characterization of hypoxia-inducible endogenous promoters in Aspergillus niger.Applied microbiology and biotechnology · 2021Article
- Cell-based and cell-free biocatalysis for the production of D-glucaric acid.Biotechnology for biofuels · 2020Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
objectiveTo enhance the glucaric acid (GA) production in Saccharomyces cerevisiae, the Vitreoscilla hemoglobin was employed to reinforce cellular oxygen supplement. Additionally, the pH-free fermentation strategy was engaged to lower the cost brought by base feeding during the acid-accumulated and long-period glucaric acid production.
resultsRecombinant yeast Bga-4 was constructed harboring Vitreoscilla hemoglobin on the basis of previous Bga-3. Higher glucose uptake rate, growth rate, and ethanol reuse rate were achieved in Bga-4 in shake-flask fermentation than those in Bga-3. Furthermore, the fed-batch fermentation in a 5-L bioreactor was performed without pH control, resulting in a final glucaric acid titer of 6.38 g/L.
conclusionsBoth the GA titer and biomass were enhanced along with the efficiency of ethanol re-utilization in the presence of VHb. Moreover, the absence of base feeding for long-period fermentation reduced production cost, which is meaningful for industrial applications.
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Registered trials
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