ArticleNature plants2020
Degradation of SERRATE via ubiquitin-independent 20S proteasome to survey RNA metabolism.
Article in Nature plants, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- Article
- SERRATE drives phase separation behaviours to regulate m6A modification and miRNA biogenesis.Nature cell biology · 2024Article
- RNA helicase Brr2a promotes miRNA biogenesis by properly remodelling secondary structure of pri-miRNAs.Nature plants · 2024Article
- Proteolytic control of the RNA silencing machinery.The Plant cell · 2024Review
- Parallel degradome-seq and DMS-MaPseq substantially revise the miRNA biogenesis atlas in Arabidopsis.Nature plants · 2024Article
- The spliceosome-associated protein CWC15 promotes miRNA biogenesis in Arabidopsis.Nature communications · 2024Article
- How intrinsically disordered proteins order plant gene silencing.Trends in genetics : TIG · 2024Review
- The chromatin remodeller MdRAD5B enhances drought tolerance by coupling MdLHP1-mediated H3K27me3 in apple.Plant biotechnology journal · 2024Article
- Structured 3' UTRs destabilize mRNAs in plants.Genome biology · 2024Article
- JANUS, a spliceosome-associated protein, promotes miRNA biogenesis in Arabidopsis.Nucleic acids research · 2024Article
- Capture of regulatory factors via CRISPR-dCas9 for mechanistic analysis of fine-tuned SERRATE expression in Arabidopsis.Nature plants · 2024Article
- microRNA biogenesis and stabilization in plants.Fundamental research · 2023Review
- Review
- Functions and mechanisms of RNA helicases in plants.Journal of experimental botany · 2023Review
- Intrinsically disordered proteins SAID1/2 condensate on SERRATE for dual inhibition of miRNA biogenesis in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The plant FYVE domain-containing protein FREE1 associates with microprocessor components to repress miRNA biogenesis.EMBO reports · 2023Article
- microRNA production inFrontiers in plant science · 2023Review
- Proteasome Dysfunction Leads to Suppression of the Hypoxic Response Pathway in Arabidopsis.International journal of molecular sciences · 2022Article
- SlRBP1 promotes translational efficiency via SleIF4A2 to maintain chloroplast function in tomato.The Plant cell · 2022Article
- PRP4KA phosphorylates SERRATE for degradation via 20Science advances · 2022Article
Corrections and comments
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Authors and funding
15 authors at 5 institutions in 2 countries.
Funding
Abstract
SERRATE (SE) is a key factor in RNA metabolism. Here, we report that SE binds 20S core proteasome α subunit G1 (PAG1) among other components and is accumulated in their mutants. Purified PAG1-containing 20S proteasome degrades recombinant SE via an ATP- and ubiquitin-independent manner in vitro. Nevertheless, PAG1 is a positive regulator for SE in vivo, as pag1 shows comparable molecular and/or developmental defects relative to se. Furthermore, SE is poorly assembled into macromolecular complexes, exemplified by the microprocessor in pag1 compared with Col-0. SE overexpression triggered the destruction of both transgenic and endogenous protein, leading to similar phenotypes of se and SE overexpression lines. We therefore propose that PAG1 degrades the intrinsically disordered portion of SE to secure the functionality of folded SE that is assembled and protected in macromolecular complexes. This study provides insight into how the 20S proteasome regulates RNA metabolism through controlling its key factor in eukaryotes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.