ArticleNature nanotechnology2020
Programming multi-protein assembly by gene-brush patterns and two-dimensional compartment geometry.
Article in Nature nanotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
- Autonomous biogenesis of all thirty proteins of the Escherichia coli translation machinery.Nature communications · 2025Article
- Delay-facilitated self-assembly in compartmentalized systems.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Bacterial chromosome conformation and cell-free gene expression in synthetic 2D compartments.Nature communications · 2025Article
- Reconstituting alternative life using the test-bed of cell-free systems.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Large-scale-integration and collective oscillations of 2D artificial cells.Nature communications · 2024Article
- PHEIGES: all-cell-free phage synthesis and selection from engineered genomes.Nature communications · 2024Article
- A genetic circuit on a single DNA molecule as an autonomous dissipative nanodevice.Nature communications · 2024Article
- Computational analysis of protein synthesis, diffusion, and binding in compartmental biochips.Microbial cell factories · 2023Article
- Toward Memory in a DNA Brush: Site-Specific Recombination Responsive to Polymer Density, Orientation, and Conformation.Journal of the American Chemical Society · 2023Article
- Solid-Phase Cell-Free Protein Synthesis and Its Applications in Biotechnology.Advances in biochemical engineering/biotechnology · 2023Article
- Cell-Free Gene Expression from DNA Brushes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Superstructured mesocrystals through multiple inherent molecular interactions for highly reversible sodium ion batteries.Science advances · 2021Article
- Boundary-Free Ribosome Compartmentalization by Gene Expression on a Surface.ACS synthetic biology · 2021Article
- The all-E. coliTXTL toolbox 3.0: new capabilities of a cell-free synthetic biology platform.Synthetic biology (Oxford, England) · 2021Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The assembly of protein machines in cells is precise, rapid, and coupled to protein synthesis with regulation in space and time. The assembly of natural and synthetic nanomachines could be similarly controlled by genetic programming outside the cell. Here, we present quasi-two-dimensional (2D) silicon compartments that enable programming of protein assembly lines by local synthesis from surface-immobilized DNA brushes. Using this platform, we studied the autonomous synthesis and assembly of a structural complex from a bacteriophage and a bacterial RNA-synthesizing machine. Local synthesis and surface capture of complexes provided high assembly yield and sensitive detection of spatially resolved assembly intermediates, with the 3D geometry of the compartment and the 2D pattern of brushes dictating the yield and mode of assembly steps. Localized synthesis of proteins in a single gene brush enhances their interactions, and displacement of their genes in separated brushes leads to step-by-step surface assembly. This methodology enables spatial regulation of protein synthesis, and deciphering, reconstruction and design of biological machine assembly lines.
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