ArticleProceedings of the National Academy of Sciences of the United States of America2020
Bidirectional titration of yeast gene expression using a pooled CRISPR guide RNA approach.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- High-throughput metabolic engineering ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Unraveling the regulatory dynamics of bidirectional promoters for modulating gene co-expression and metabolic flux in Saccharomyces cerevisiae.Nucleic acids research · 2025Article
- Recent advances in genetic engineering and chemical production in yeast species.FEMS yeast research · 2025Review
- Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit.Nature communications · 2024Article
- Application of genome editing techniques to regulate gene expression in crops.BMC plant biology · 2024Review
- Coupling High-Throughput and Targeted Screening for Identification of Nonobvious Metabolic Engineering Targets.ACS synthetic biology · 2024Article
- Engineered upstream open reading frames predictably downregulate mRNA translation in plants.Nature biotechnology · 2023Article
- Tuning plant phenotypes by precise, graded downregulation of gene expression.Nature biotechnology · 2023Article
- Titrating Avidity of Yeast-Displayed Proteins Using a Transcriptional Regulator.ACS synthetic biology · 2023Article
- Genome-Wide Screen for Enhanced Noncanonical Amino Acid Incorporation in Yeast.ACS synthetic biology · 2022Article
- Biosensor-CoupledmSystems · 2022Article
- High-content CRISPR screening.Nature reviews. Methods primers · 2022Article
- Dynamic Progress in Technological Advances to Study Lipids in Aging: Challenges and Future Directions.Frontiers in aging · 2022Review
- Engineering the bilayer: Emerging genetic tool kits for mechanistic lipid biology.Current opinion in chemical biology · 2021Review
- Intelligent host engineering for metabolic flux optimisation in biotechnology.The Biochemical journal · 2021Review
- Sorting for secreted molecule production using a biosensor-in-microdroplet approach.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Synthetic Lethality in Cancer Therapeutics: The Next Generation.Cancer discovery · 2021Review
- A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast.BMC genomics · 2021Article
- A simplified strategy for titrating gene expression reveals new relationships between genotype, environment, and bacterial growth.Nucleic acids research · 2021Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most classic genetic approaches utilize binary modifications that preclude the identification of key knockdowns for essential genes or other targets that only require moderate modulation. As a complementary approach to these classic genetic methods, we describe a plasmid-based library methodology that affords bidirectional, graded modulation of gene expression enabled by tiling the promoter regions of all 969 genes that comprise the ito977 model of
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.