ArticleFluids and barriers of the CNS2020
Preterm intraventricular hemorrhage in vitro: modeling the cytopathology of the ventricular zone.
Article in Fluids and barriers of the CNS, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- In Vitro Assessment of Ventricular Catheters with a Multilayered Fibrous Web to Prevent Cellular Occlusion.ACS biomaterials science & engineering · 2026Article
- Subventricular Zone-on-a-Chip: A Model to Study Neurogenesis Disruption in Neonatal Intraventricular Hemorrhage.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Research priorities for improving cognitive and neuropsychological outcomes in hydrocephalus.Fluids and barriers of the CNS · 2024Review
- Early depletion of gut microbiota shape oligodendrocyte response after traumatic brain injury.Journal of neuroinflammation · 2024Article
- Gut Microbiota Shape Oligodendrocyte Response after Traumatic Brain Injury.Research square · 2024Article
- Intraventricular dimethyl sulfoxide (DMSO) induces hydrocephalus in a dose-dependent pattern.Heliyon · 2024Article
- Polyvinylpyrrolidone-coated catheters decrease choroid plexus adhesion and improve flow/pressure performance in an in vitro model of hydrocephalus.Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery · 2024Article
- Recombinant Erythropoietin Induces Oligodendrocyte Progenitor Cell Proliferation After Traumatic Brain Injury and Delayed Hypoxemia.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2023Article
- Generation of Periventricular Reactive Astrocytes Overexpressing Aquaporin 4 Is Stimulated by Mesenchymal Stem Cell Therapy.International journal of molecular sciences · 2023Article
- Polyvinylpyrrolidone-Coated Catheters Decrease Astrocyte Adhesion and Improve Flow/Pressure Performance in an Invitro Model of Hydrocephalus.Children (Basel, Switzerland) · 2022Article
- AQP4, Astrogenesis, and Hydrocephalus: A New Neurological Perspective.International journal of molecular sciences · 2022Review
- Mechanisms of neuroinflammation in hydrocephalus after intraventricular hemorrhage: a review.Fluids and barriers of the CNS · 2022Review
- AQP4 labels a subpopulation of white matter-dependent glial radial cells affected by pediatric hydrocephalus, and its expression increased in glial microvesicles released to the cerebrospinal fluid in obstructive hydrocephalus.Acta neuropathologica communications · 2022Article
- Acquired hydrocephalus is associated with neuroinflammation, progenitor loss, and cellular changes in the subventricular zone and periventricular white matter.Fluids and barriers of the CNS · 2022Article
- Response of Astrocytes to Blood Exposure due to Shunt InsertionAIChE journal. American Institute of Chemical Engineers · 2021Article
- Impact of sex differences on thrombin-induced hydrocephalus and white matter injury: the role of neutrophils.Fluids and barriers of the CNS · 2021Article
- Brain Barriers and brain fluids research in 2020 and the fluids and barriers of the CNS thematic series on advances in in vitro modeling of the blood-brain barrier and neurovascular unit.Fluids and barriers of the CNS · 2021Article
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Authors and funding
6 authors.
Funding
Abstract
backgroundSevere intraventricular hemorrhage (IVH) is one of the most devastating neurological complications in preterm infants, with the majority suffering long-term neurological morbidity and up to 50% developing post-hemorrhagic hydrocephalus (PHH). Despite the importance of this disease, its cytopathological mechanisms are not well known. An in vitro model of IVH is required to investigate the effects of blood and its components on the developing ventricular zone (VZ) and its stem cell niche. To address this need, we developed a protocol from our accepted in vitro model to mimic the cytopathological conditions of IVH in the preterm infant.
methodsMaturing neuroepithelial cells from the VZ were harvested from the entire lateral ventricles of wild type C57BL/6 mice at 1-4 days of age and expanded in proliferation media for 3-5 days. At confluence, cells were re-plated onto 24-well plates in differentiation media to generate ependymal cells (EC). At approximately 3-5 days, which corresponded to the onset of EC differentiation based on the appearance of multiciliated cells, phosphate-buffered saline for controls or syngeneic whole blood for IVH was added to the EC surface. The cells were examined for the expression of EC markers of differentiation and maturation to qualitatively and quantitatively assess the effect of blood exposure on VZ transition from neuroepithelial cells to EC. DISCUSSION: This protocol will allow investigators to test cytopathological mechanisms contributing to the pathology of IVH with high temporal resolution and query the impact of injury to the maturation of the VZ. This technique recapitulates features of normal maturation of the VZ in vitro, offering the capacity to investigate the developmental features of VZ biogenesis.
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