ArticleWater research2020
Metagenomic water quality monitoring with a portable laboratory.
Article in Water research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 97 citations in OpenAlex.
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- Comparison of Environmental Microbiomes, Resistomes and Plasmidomes from a Human Tertiary Hospital and Companion Animal Veterinary Hospital in London, UK.Antibiotics (Basel, Switzerland) · 2026Article
- Integrated approaches for pathogen monitoring and shotgun metagenomic analysis in Atlantic salmon farming.Scientific reports · 2026Article
- Quantitative metagenomics using a portable protocol.Applied and environmental microbiology · 2026Article
- Significant impact of threshold adjustments on microbiome characterization following Nanopore sequencing.ISME communications · 2026Article
- Integrating environmental and health data: insights and lessons from Australia's water monitoring systems.Journal of water and health · 2025Review
- Harnessing Engineered Microbial Consortia for Xenobiotic Bioremediation: Integrating Multi-Omics and AI for Next-Generation Wastewater Treatment.Journal of xenobiotics · 2025Review
- Nanopore Environmental Analysis.JACS Au · 2025Review
- Current Progress and Future Trends of Genomics-Based Techniques for Food Adulteration Identification.Foods (Basel, Switzerland) · 2025Review
- Analysis of metagenomic data.Nature reviews. Methods primers · 2025Article
- Biosensors and Bioassays for the Medical Detection ofCurrent medicinal chemistry · 2025Review
- The need for adaptability in detection, characterization, and attribution of biosecurity threats.Nature communications · 2024Review
- Assessment of Skimmed Milk Flocculation for Bacterial Enrichment from Water Samples, and Benchmarking of DNA Extraction and 16S rRNA Databases for Metagenomics.International journal of molecular sciences · 2024Article
- Bacterial community assessment of drinking water and downstream distribution systems in highland localities of Ecuador.Journal of water and health · 2024Article
- Article
- Metagenomics: An Effective Approach for Exploring Microbial Diversity and Functions.Foods (Basel, Switzerland) · 2023Review
- Complexities of the urban drinking water systems in Ethiopia and possible interventions for sustainability.Environment, development and sustainability · 2023Article
- Evaluation of environmental factors and microbial community structure in an important drinking-water reservoir across seasons.Frontiers in microbiology · 2023Article
- Article
- Transitions in bacterial communities across two fermentation-based virgin coconut oil (VCO) production processes.Heliyon · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe the technical feasibility of metagenomic water quality analysis using only portable equipment, for example mini-vacuum pumps and filtration units, mini-centrifuges, mini-PCR machines and the memory-stick sized MinION of Oxford Nanopore Technologies, for the library preparation and sequencing of 16S rRNA gene amplicons. Using this portable toolbox on site, we successfully characterized the microbiome of water samples collected from Birtley Sewage Treatment Plant, UK, and its environs. We also demonstrated the applicability of the portable metagenomics toolbox in a low-income country by surveying water samples from the Akaki River around Addis Ababa, Ethiopia. The 16S rRNA gene sequencing workflow, including DNA extraction, PCR amplification, sequencing library preparation, and sequencing was accomplished within one working day. The metagenomic data became available within 24-72 h, depending on internet speed. Metagenomic analysis clearly distinguished the microbiome of pristine samples from sewage influenced water samples. Metagenomic analysis identified the potential role of two bacterial genera not conventionally monitored, Arcobacter and Aeromonas, as predominant faecal pollution indicators/waterborne hazards. Subsequent quantitative PCR analysis validated the high Arcobacter butzleri abundances observed in the urban influenced Akaki River water samples by portable next generation sequencing with the MinION device. Overall, our field deployable metagenomics toolbox advances the capability of scientists to comprehensively monitor microbiomes anywhere in the world, including in the water, food and drinks industries, the health services, agriculture and beyond.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.