Evidence map›Paper›PMID 32687676›Full record

ArticleCurrent protocols in protein science2020

Synthesis of Recombinant Human Hemoglobin With NH

Chandrasekhar Natarajan, Anthony V Signore, Vikas Kumar, Jay F Storz

Open access · greenAbstract read
In one paragraph

Article in Current protocols in protein science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Chandrasekhar NatarajanSchool of Biological Sciences, University of Nebraska, Lincoln, Nebraska.
Anthony V SignoreSchool of Biological Sciences, University of Nebraska, Lincoln, Nebraska.
Vikas KumarMass Spectrometry and Proteomics Core Facility, University of Nebraska Medical Center, Omaha, Nebraska.
Jay F StorzSchool of Biological Sciences, University of Nebraska, Lincoln, Nebraska.
University of Nebraska System · USNebraska Medical Center · USUniversity of Nebraska–Lincoln · US

Funding

Mutational Pleiotropy, Epistasis, and the Adaptive Evolution of Hemoglobin FunctionR01HL087216 · NHLBI · UNIVERSITY OF NEBRASKA LINCOLN · PI STORZ, JAY · 2008 to 2021
$4.5M
NHLBI NIH HHS R01 HL087216
6 · The paper itself

Abstract

The development of new technologies for the efficient expression of recombinant hemoglobin (rHb) is of interest for experimental studies of protein biochemistry and the development of cell-free blood substitutes in transfusion medicine. Expression of rHb in Escherichia coli host cells has numerous advantages, but one disadvantage of using prokaryotic systems to express eukaryotic proteins is that they are incapable of performing post-translational modifications such as NH

Indexed as

Protein Processing, Post-TranslationalAcetylationalpha-GlobinsBase Sequencebeta-GlobinsDNA Restriction EnzymesEscherichia coliGene ExpressionHemoglobinsHumansPlasmidsRecombinant Proteinsalpha-Globinsbeta-GlobinsDNA Restriction EnzymesHemoglobinsRecombinant Proteinsblood substituteNH2-terminal acetylationpost-translational modificationrecombinant hemoglobin

Identifiers

PMID32687676
PMCPMC7505543
OpenAlexW3043425167

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.