ArticlePloS one2020
Stability of β-lactam antibiotics in bacterial growth media.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 49 citations in OpenAlex.
- Developing bacterial resistance to antibiotics: a laboratory activity.Access microbiology · 2026Article
- Nanostructured Dual-Delivery System with Antioxidant and Synergistic Approach for Targeted Dermal Treatment.International journal of molecular sciences · 2025Article
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- Assessment of Biodegradation Mechanisms of Ceftiofur Sodium byMicroorganisms · 2025Article
- ESKAPE pathogens rapidly develop resistance against antibiotics in development in vitro.Nature microbiology · 2025Article
- Antibacterial and antibiofilm activities of kaffir lime essential oils and their active constituents againstHeliyon · 2025Article
- Article
- Bacterial aggregation triggered by low-level antibiotic-mediated lysis.NPJ biofilms and microbiomes · 2024Article
- Optimisation of analytical methods for tuberculosis drug detection in wastewater: A multinational study.Heliyon · 2024Article
- Amoxicillin/clavulanate in combination with rifampicin/clarithromycin is bactericidal against Mycobacterium ulcerans.PLoS neglected tropical diseases · 2024Article
- Comprehensive stability analysis of 13 β-lactams and β-lactamase inhibitors inAntimicrobial agents and chemotherapy · 2024Article
- Self-assembled silk fibroin cross-linked with genipin supplements microbial carbonate precipitation in building material.Environmental microbiology reports · 2023Article
- A novel coli myophage and antibiotics synergistically inhibit the growth of the uropathogenicMicrobiology spectrum · 2023Article
- Ribosome-binding antibiotics increase bacterial longevity and growth efficiency.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Evaluation of a Tetracycline-ResistantPathogens (Basel, Switzerland) · 2023Article
- Environmental conditions define the energetics of bacterial dormancy and its antibiotic susceptibility.Biophysical journal · 2023Article
- Ampicillin Stability in a Portable Elastomeric Infusion Pump: A Step Forward in Outpatient Parenteral Antimicrobial Therapy.Pharmaceutics · 2023Article
- Novel Synergies and Isolate Specificities in the Drug Interaction Landscape of Mycobacterium abscessus.Antimicrobial agents and chemotherapy · 2023Article
- Article
- Widening the net: a literature review of antimicrobial agents with potential suitability for outpatient parenteral antimicrobial therapy services-the importance of storage and stability.European journal of hospital pharmacy : science and practice · 2023Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laboratory assays such as MIC tests assume that antibiotic molecules are stable in the chosen growth medium-but rapid degradation has been observed for antibiotics including β-lactams under some conditions in aqueous solution. Degradation rates in bacterial growth medium are less well known. Here, we develop a 'delay time bioassay' that provides a simple way to estimate antibiotic stability in bacterial growth media, using only a plate reader and without the need to measure the antibiotic concentration directly. We use the bioassay to measure degradation half-lives of the β-lactam antibiotics mecillinam, aztreonam and cefotaxime in widely-used bacterial growth media based on MOPS and Luria-Bertani (LB) broth. We find that mecillinam degradation can occur rapidly, with a half-life as short as 2 hours in MOPS medium at 37°C and pH 7.4, and 4-5 hours in LB, but that adjusting the pH and temperature can increase its stability to a half-life around 6 hours without excessively perturbing growth. Aztreonam and cefotaxime were found to have half-lives longer than 6 hours in MOPS medium at 37°C and pH 7.4, but still shorter than the timescale of a typical minimum inhibitory concentration (MIC) assay. Taken together, our results suggest that care is needed in interpreting MIC tests and other laboratory growth assays for β-lactam antibiotics, since there may be significant degradation of the antibiotic during the assay.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.