Evidence map›Paper›PMID 32687193›Full record

ArticleNucleic acids research2020

Single-molecule live-cell imaging reveals RecB-dependent function of DNA polymerase IV in double strand break repair.

Sarah S Henrikus, Camille Henry, Amy E McGrath, Slobodan Jergic, John P McDonald, Yvonne Hellmich, Steven T Bruckbauer, Matthew L Ritger, Megan E Cherry, Elizabeth A Wood and 7 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 17 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 5 institutions in 3 countries.

Sarah S HenrikusMolecular Horizons Institute and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.
Camille HenryDepartment of Biochemistry, University of Wisconsin-Madison, WI 53706, USA.
Amy E McGrathMolecular Horizons Institute and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.
Slobodan JergicMolecular Horizons Institute and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.
John P McDonaldLaboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Yvonne HellmichInstitute of Biochemistry, Goethe Universität, Frankfurt 3MR4+W2, Germany.
Steven T BruckbauerDepartment of Biochemistry, University of Wisconsin-Madison, WI 53706, USA.
Matthew L RitgerDepartment of Biochemistry, University of Wisconsin-Madison, WI 53706, USA.
Megan E CherryMolecular Horizons Institute and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.
Elizabeth A WoodDepartment of Biochemistry, University of Wisconsin-Madison, WI 53706, USA.
Phuong T PhamDepartment of Biological Sciences, University of Southern California, Los Angeles, CA 90089, USA.
Myron F GoodmanDepartments of Biological Sciences and Chemistry, University of Southern California, Los Angeles, CA 90089, USA.
Roger WoodgateLaboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Michael M CoxDepartment of Biochemistry, University of Wisconsin-Madison, WI 53706, USA.
Antoine M van OijenMolecular Horizons Institute and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.
Harshad GhodkeMolecular Horizons Institute and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.
Andrew RobinsonMolecular Horizons Institute and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.
University of Wollongong · AUUniversity of Wisconsin–Madison · USNational Institutes of Health · USUniversity of Southern California · USGoethe University Frankfurt · DE

Funding

DNA Replication, Repair, and Mutagenesis In Eukaryotic And Prokaryotic CellsZIAHD001500 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI WOODGATE, ROGER · 2009 to 2025
$37.5M
Creation and Repair of Postreplicative DNA GapsRM1GM130450 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Michael M. Cox, MYRON GOODMAN · 2019 to 2026
$10.4M
THE BIOCHEMISTRY OF GENETIC RECOMBINATION/RECA PROTEINR01GM032335 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI COX, MICHAEL M. · 1985 to 2014
$6.8M
Hypermutation in Bacteria and HumansR35ES028343 · NIEHS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI GOODMAN, MYRON · 2017 to 2024
$4.3M
Mechanisms and applications of recombinational DNA repairU01GM032335 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI COX, MICHAEL M. · 2015 to 2018
$1.7M
NIEHS NIH HHS R35 ES028343NIGMS NIH HHS R01 GM032335NIGMS NIH HHS RM1 GM130450NIGMS NIH HHS U01 GM032335
6 · The paper itself

Abstract

Several functions have been proposed for the Escherichia coli DNA polymerase IV (pol IV). Although much research has focused on a potential role for pol IV in assisting pol III replisomes in the bypass of lesions, pol IV is rarely found at the replication fork in vivo. Pol IV is expressed at increased levels in E. coli cells exposed to exogenous DNA damaging agents, including many commonly used antibiotics. Here we present live-cell single-molecule microscopy measurements indicating that double-strand breaks induced by antibiotics strongly stimulate pol IV activity. Exposure to the antibiotics ciprofloxacin and trimethoprim leads to the formation of double strand breaks in E. coli cells. RecA and pol IV foci increase after treatment and exhibit strong colocalization. The induction of the SOS response, the appearance of RecA foci, the appearance of pol IV foci and RecA-pol IV colocalization are all dependent on RecB function. The positioning of pol IV foci likely reflects a physical interaction with the RecA* nucleoprotein filaments that has been detected previously in vitro. Our observations provide an in vivo substantiation of a direct role for pol IV in double strand break repair in cells treated with double strand break-inducing antibiotics.

Indexed as

CiprofloxacinDNA-Binding ProteinsDNA Breaks, Double-StrandedDNA DamageDNA Polymerase betaDNA RepairDNA ReplicationEscherichia coliEscherichia coli ProteinsExodeoxyribonuclease VRec A RecombinasesSingle Molecule ImagingCiprofloxacinDNA-Binding ProteinsDNA Polymerase betaEscherichia coli ProteinsExodeoxyribonuclease Vexodeoxyribonuclease V, E colirecA protein, E coliRec A Recombinases

Identifiers

PMID32687193
PMCPMC7470938
OpenAlexW3044617970

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.