ArticleAmerican journal of clinical pathology2020
Distribution of SARS-CoV-2 PCR Cycle Threshold Values Provide Practical Insight Into Overall and Target-Specific Sensitivity Among Symptomatic Patients.
Article in American journal of clinical pathology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 78 citations in OpenAlex.
- Viral dynamics and factors associated with duration of COVID-19 positivity: evidence from the first-three epidemiological waves in Cameroon.BMC infectious diseases · 2025Article
- Comparative Analysis of Viral Load and Cytokines during SARS-CoV-2 Infection between Pregnant and Non-Pregnant Women.International journal of molecular sciences · 2024Article
- Ct,Future science OA · 2023Article
- Post-COVID-19 Syndrome in Outpatients and Its Association with Viral Load.International journal of environmental research and public health · 2022Article
- The Relationship Between Population-Level SARS-CoV-2 Cycle Threshold Values and Trend of COVID-19 Infection: Longitudinal Study.JMIR public health and surveillance · 2022Article
- Clinical Profiles at the Time of Diagnosis of SARS-CoV-2 Infection in Costa Rica During the Pre-vaccination Period Using a Machine Learning Approach.Phenomics (Cham, Switzerland) · 2022Article
- Potential risk of SARS-CoV-2 infection among people handling linens used by COVID-19 patients before and after washing.Scientific reports · 2022Article
- Relevance of cycle threshold values in mass screening by reverse-transcription-PCR during COVID-19 pandemic in Belgium: a decision-making support?Future virology · 2022Article
- Elucidation of correlation between SARS-CoV-2 RdRp and N gene cycle threshold (Ct) by RT-PCR with age and gender.Clinica chimica acta; international journal of clinical chemistry · 2022Article
- CRISPR-based point-of-care diagnostics incorporating Cas9, Cas12, and Cas13 enzymes advanced for SARS-CoV-2 detection.Journal of biochemical and molecular toxicology · 2022Review
- Rapid, adaptable and sensitive Cas13-based COVID-19 diagnostics using ADESSO.Nature communications · 2022Article
- The utility of rapid FIA antigen test to detect SARS-CoV-2 infection - preliminary experiences of the Hospital for Infectious Diseases in Warsaw.Postepy dermatologii i alergologii · 2022Article
- COVID-19 Cycle Threshold/Cycle Number Testing at a Community Living Center.Federal practitioner : for the health care professionals of the VA, DoD, and PHS · 2022Article
- A random priming amplification method for whole genome sequencing of SARS-CoV-2 virus.BMC genomics · 2022Article
- Epidemiological, virological and clinical features of SARS-CoV-2 among individuals during the first wave in Cameroon: Baseline analysis for the EDCTP PERFECT-Study RIA2020EF-3000.Journal of public health in Africa · 2022Article
- Effectiveness and use of reverse transcriptase polymerase chain reaction point of care testing in a large-scale COVID-19 surveillance system.Pharmacoepidemiology and drug safety · 2022Article
- Evaluation of efficiency and sensitivity of 1D and 2D sample pooling strategies for SARS-CoV-2 RT-qPCR screening purposes.Scientific reports · 2022Article
- Importance of sample input volume for accurate SARS-CoV-2 qPCR testing.Analytica chimica acta · 2022Article
- Increased complications in patients who test COVID-19 positive after elective surgery and implications for pre and postoperative screening.American journal of surgery · 2022Article
- SARS-CoV-2 viral-load distribution reveals that viral loads increase with age: a retrospective cross-sectional cohort study.International journal of epidemiology · 2022Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesWe examined the distribution of reverse transcription polymerase chain reaction (RT-PCR) cycle threshold (CT) values obtained from symptomatic patients being evaluated for coronavirus disease 2019 (COVID-19) to determine the proportion of specimens containing a viral load near the assay limit of detection (LoD) to gain practical insight to the risk of false-negative results. We also examined the relationship between CT value and patient age to determine any age-dependent difference in viral load or test sensitivity.
methodsWe collected CT values obtained from the cobas severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) assay corresponding to 1,213 combined nasopharyngeal-oropharyngeal specimens obtained from symptomatic individuals that were reported as positive or presumptive positive for SARS-CoV-2. CT values were stratified by SARS-CoV target and patient age group.
resultsIn total, 93.3% to 98.4% of specimens demonstrated CT values greater than 3× the assay LoD, at which point false-negative results would not be expected. The mean of CT values between age groups was statistically equivalent with the exception of patients in age group 80 to 89 years, which demonstrated slightly lower CTs.
conclusionsBased on the distribution of observed CT values, including the small proportion of specimens with values near the assay LoD, there is a low risk of false-negative RT-PCR results in combined nasopharyngeal-oropharyngeal specimens obtained from symptomatic individuals.
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