ArticleIranian journal of microbiology2020
Comparison five primer sets from different genome region of COVID-19 for detection of virus infection by conventional RT-PCR.
Article in Iranian journal of microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- The effect of sample site and collection procedure on identification of SARS-CoV-2 infection.The Cochrane database of systematic reviews · 2024Pooled it
- Diagnostic value comparative analysis of the commercial kits for the detection of SARS-CoV-2 in clinical samples: a systematic review and meta-analysis.Iranian journal of microbiology · 2025Review
- Rapid detection of SARS-CoV-2 RNA using a one-step fast multiplex RT-PCR coupled to lateral flow immunoassay.BMC infectious diseases · 2024Article
- Disappearance of Imported Cases of Omicron Lineage BA.2.40 in West Kalimantan, Indonesia.Iranian journal of medical sciences · 2024Article
- Clinical Characteristics, Diagnosis, and Therapeutics of COVID-19: A Review.Current medical science · 2023Review
- The Diagnostic Performance of Various Clinical Specimens for the Detection of COVID-19: A Meta-Analysis of RT-PCR Studies.Diagnostics (Basel, Switzerland) · 2023Review
- Mathematical modeling for Delta and Omicron variant of SARS-CoV-2 transmission dynamics in Greece.Infectious Disease Modelling · 2023Article
- Performances of four Nucleic Acid Amplification Tests for the identification of SARS-CoV-2 in Ethiopia.Scientific reports · 2022Article
- Identification of mutations in SARS-CoV-2 PCR primer regions.Scientific reports · 2022Article
- Miniaturized Real-Time PCR systems for SARS-CoV-2 detection at the Point-of-Care.Clinica chimica acta; international journal of clinical chemistry · 2022Review
- Review
- Research progress in laboratory detection of SARS-CoV-2.Irish journal of medical science · 2022Review
- Real-time PCR Ct value in SARS-CoV-2 detection: RdRp or N gene?Infection · 2022Article
- Article
- Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR.Applied microbiology and biotechnology · 2022Article
- Considerations about Reducing False-Negative PCR Test for COVID-19.International journal of MCH and AIDS · 2022Article
- Influenza A, Influenza B, and SARS-CoV-2 Similarities and Differences - A Focus on Diagnosis.Frontiers in microbiology · 2022Review
- Article
- rRT-PCR for SARS-CoV-2: Analytical considerations.Clinica chimica acta; international journal of clinical chemistry · 2021Review
- Biological characteristics and biomarkers of novel SARS-CoV-2 facilitated rapid development and implementation of diagnostic tools and surveillance measures.Biosensors & bioelectronics · 2021Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectivesThe new beta-coronavirus, which caused Severe Acute Respiratory Coronavirus-2 Syndrome (SARS-CoV-2), a major respiratory outbreak in Wuhan, China in December 2019, is now prevalent in many countries around the world. Identifying PCR-based viruses is a well-known and relatively stable protocol. Unfortunately, the high mutation rates may lead to widespread changes in viral nucleic acid sequences, and so using specific primers for PCR can be recommended. In this study, we evaluated the power of a conventional RT-PCR to detect SARS-CoV-2 RNA among the five set primer sets. MATERIALS AND
methodsThe five genomic regions of the Coronavirus SARS-2 virus including Nucleocapsids (N), Envelope (E), RNA depended RNA Polymerase (RdRp), ORF1ab and Spike (S) were selected for primer designing. A conventional RT-PCR was performed to compare sensitivity, specificity and other analytical characteristics of primers designed against two Real Time PCR commercial kits.
resultsThe result of the comparative analysis showed that the ORF1ab, N and RdRp primers had a sensitivity, specificity and positive predictive value higher than other primers. A significant difference in the analytical sensitivity between the studied primer sets in RT-PCR kits was observed.
conclusionIn this study, the ORF1ab, Nucleocapsid and RdRp regions have the best primers for identifying the SARS-CoV-2 RNA between different genes that have been suggested.
Indexed as
Identifiers
32685113PMC7340604W3033975296What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.