ArticleiScience2020
Biosynthesis of Circular RNA ciRS-7/CDR1as Is Mediated by Mammalian-wide Interspersed Repeats.
Article in iScience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 38 citations in OpenAlex.
- Impact ofJournal of cardiovascular development and disease · 2026Review
- SAM68 is a multifunctional post-transcriptional regulator of cardiomyocyte differentiation.Nucleic acids research · 2026Article
- The history and function of a circular RNA.Nature communications · 2026Review
- Circular RNAs as Biomarkers and Therapeutic Targets in Diabetic Cardiovascular Complications.Endocrinology, diabetes & metabolism · 2026Review
- Circular RNAs: from transcriptional noise to engineered therapeutics.Frontiers in epigenetics and epigenomics · 2026Review
- Molecular basis of backsplicing regulation and its application to manipulate circRNA levels.RNA biology · 2025Review
- ciRS-7 expression is epigenetically regulated in cancer cells across human adenocarcinomas.PLoS genetics · 2025Article
- Article
- Noncoding RNAs and their influence on maternal mental health: insights into perinatal depression: a review.Molecular biology reports · 2025Review
- Non-coding RNAs: emerging biomarkers and therapeutic targets in cancer and inflammatory diseases.Frontiers in oncology · 2025Review
- Review
- Utility of oligonucleotide in upregulating circular RNA production in a cellular model.Scientific reports · 2024Article
- Perspectives of circular RNAs in diabetic complications from biological markers to potential therapeutic targets (Review).Molecular medicine reports · 2023Article
- Article
- Functions of Circular RNA in Human Diseases and Illnesses.Non-coding RNA · 2023Review
- circROBO1 promotes prostate cancer growth and enzalutamide resistance via accelerating glycolysis.Journal of Cancer · 2023Article
- circRNA: Regulatory factors and potential therapeutic targets in inflammatory dermatoses.Journal of cellular and molecular medicine · 2022Review
- Circ_SAR1A regulates the malignant behavior of lung cancer cells via the miR-21-5p/TXNIP axis.Journal of clinical laboratory analysis · 2022Article
- The potential of using blood circular RNA as liquid biopsy biomarker for human diseases.Protein & cell · 2021Review
- Noncoding RNA crosstalk in brain health and diseases.Neurochemistry international · 2021Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Circular RNAs (circRNAs) are stable non-coding RNAs with a closed circular structure. One of the best studied circRNAs is ciRS-7 (CDR1as), which acts as a regulator of the microRNA miR-7; however, its biosynthetic pathway has remained an enigma. Here we delineate the biosynthetic pathway of ciRS-7. The back-splicing events that form circRNAs are often facilitated by flanking inverted repeats of the primate-specific Alu elements. The ciRS-7 gene lacks these elements, but, instead, we identified a set of flanking inverted elements belonging to the mammalian-wide interspersed repeat (MIR) family. Splicing reporter assays in HEK293 cells demonstrated that these inverted MIRs are required to generate ciRS-7 through back-splicing, and CRISPR/Cas9-mediated deletions confirmed the requirement of the endogenous MIR elements in SH-SY5Y cells. Using bioinformatic searches, we identified several other MIR-dependent circRNAs and confirmed them experimentally. We propose that MIR-mediated RNA circularization is used to generate a subset of mammalian circRNAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.