ArticleScientific reports2020
Platelet-primed interactions of coagulation and anticoagulation pathways in flow-dependent thrombus formation.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- Fibrin-bound thrombin determines clot structure and blood thrombogenicity in normofibrinogenemia and dysfibrinogenemia.Haematologica · 2026Article
- Automated Imaging and Analysis of Platelet, Coagulation and Fibrinolysis Activities Using a Novel Flow Chip-Based System at Physiological Temperature.Micromachines · 2025Article
- Rapid-Onset Venous Thrombosis After Tibia Fracture: A Case Report.The American journal of case reports · 2025Article
- Silkworm Cocoon-Derived Carbon Dots for Post-Trauma Hemostasis and Tissue Repair.Pharmaceuticals (Basel, Switzerland) · 2025Article
- Neutrophils: Linking Inflammation to Thrombosis and Unlocking New Treatment Horizons.International journal of molecular sciences · 2025Review
- Advancing Platelet Research Through Live-Cell Imaging: Challenges, Techniques, and Insights.Sensors (Basel, Switzerland) · 2025Review
- Fisetin Alleviates Inflammation and Oxidative Stress in Deep Vein Thrombosis via MAPK and NRF2 Signaling Pathway.International journal of molecular sciences · 2024Article
- Case Report: Laboratory detection of a thrombotic tendency in a family with hypodysfibrinogenemia and a novelFrontiers in cardiovascular medicine · 2024Article
- Neutrophils: a key component in ECMO-related acute organ injury.Frontiers in immunology · 2024Review
- Single molecule studies of dynamic platelet interactions with endothelial cells.Frontiers in bioengineering and biotechnology · 2024Article
- Crucial roles of red blood cells and platelets in whole blood thrombin generation.Blood advances · 2023Article
- Platelet glycoprotein V spatio-temporally controls fibrin formation.Nature cardiovascular research · 2023Article
- Reversing direct factor Xa or thrombin inhibitors: Factor V addition to prothrombin complex concentrate is beneficial in vitro.Research and practice in thrombosis and haemostasis · 2022Article
- Protein C or Protein S deficiency associates with paradoxically impaired platelet-dependent thrombus and fibrin formation under flow.Research and practice in thrombosis and haemostasis · 2022Article
- Temporal Roles of Platelet and Coagulation Pathways in Collagen- and Tissue Factor-Induced Thrombus Formation.International journal of molecular sciences · 2021Article
- Platelet GPVI (Glycoprotein VI) and Thrombotic Complications in the Venous System.Arteriosclerosis, thrombosis, and vascular biology · 2021Review
- High fibrinogen γ' levels in patient plasma increase clot formation at arterial and venous shear.Blood advances · 2021Article
- Osteonecrosis of the Femoral Head in Patients with Hypercoagulability-From Pathophysiology to Therapeutic Implications.International journal of molecular sciences · 2021Review
- Asymmetrical Forces Dictate the Distribution and Morphology of Platelets in Blood Clots.Cells · 2021Article
- Inhibition of platelet adhesion, thrombus formation, and fibrin formation by a potent αIIbβ3 integrin inhibitor from ticks.Research and practice in thrombosis and haemostasis · 2021Article
Corrections and comments
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Authors and funding
17 authors at 6 institutions in 5 countries.
Funding
Abstract
In haemostasis and thrombosis, platelet, coagulation and anticoagulation pathways act together to produce fibrin-containing thrombi. We developed a microspot-based technique, in which we assessed platelet adhesion, platelet activation, thrombus structure and fibrin clot formation in real time using flowing whole blood. Microspots were made from distinct platelet-adhesive surfaces in the absence or presence of tissue factor, thrombomodulin or activated protein C. Kinetics of platelet activation, thrombus structure and fibrin formation were assessed by fluorescence microscopy. This work revealed: (1) a priming role of platelet adhesion in thrombus contraction and subsequent fibrin formation; (2) a surface-independent role of tissue factor, independent of the shear rate; (3) a mechanism of tissue factor-enhanced activation of the intrinsic coagulation pathway; (4) a local, suppressive role of the anticoagulant thrombomodulin/protein C pathway under flow. Multiparameter analysis using blood samples from patients with (anti)coagulation disorders indicated characteristic defects in thrombus formation, in cases of factor V, XI or XII deficiency; and in contrast, thrombogenic effects in patients with factor V-Leiden. Taken together, this integrative phenotyping approach of platelet-fibrin thrombus formation has revealed interaction mechanisms of platelet-primed key haemostatic pathways with alterations in patients with (anti)coagulation defects. It can help as an important functional add-on whole-blood phenotyping.
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