ArticleParasites & vectors2020
A new protocol for absolute quantification of haemosporidian parasites in raptors and comparison with current assays.
Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 33 citations in OpenAlex.
- Prevalence and Genetic Diversity of Haemoproteus and Leucocytozoon in Raptors and Other Captive Birds at the National Zoological Garden in South Africa.Integrative zoology · 2026Article
- Diversity of haemosporidian parasites in cranes: description of Haemoproteus balearicae and its phylogenetic position within the H. antigonis clade.Parasite (Paris, France) · 2025Article
- Differences in haemosporidian parasite prevalence and diversity in migratory and resident birds of prey species revealed by a non-invasive sampling method.Ecology and evolution · 2024Article
- Molecular investigation of Haemoproteus and Plasmodium species of some raptors in Hatay province: new CytB lineages for raptors of Accipitriformes in Turkey.Parasitology research · 2023Article
- Surveillance of avian malaria and related haemoparasites in common terns (Parasitology · 2023Article
- Digital PCR: modern solution to parasite diagnostics and population trait genetics.Parasites & vectors · 2023Review
- Blood parasites (Trypanosoma, Leucocytozoon, Haemoproteus) in the Eurasian sparrowhawk (Accipiter nisus): diversity, incidence and persistence of infection at the individual level.Parasites & vectors · 2023Article
- A newly developed droplet digital PCR for Ehrlichia canis detection: comparisons to conventional PCR and blood smear techniques.The Journal of veterinary medical science · 2022Article
- Avian haemosporidian parasites of accipitriform raptors.Malaria journal · 2022Article
- Neglected parasite reservoirs in wetlands: Prevalence and diversity of avian haemosporidians in waterbird communities in Northeast China.International journal for parasitology. Parasites and wildlife · 2021Article
- Review
- Prevalence and genetic diversity of avian haemosporidian parasites in wild bird species of the order Columbiformes.Parasitology research · 2021Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundAccurate quantification of infection intensity is essential to estimate infection patterns of avian haemosporidian parasites in order to understand the evolution of host-parasite associations. Traditional microscopy is cost-effective but requires high-quality blood smears and considerable experience, while the widely used semi-quantitative qPCR methods are mostly employed with ideal, laboratory-based golden samples and standard curves, which may limit the comparison of parasitemia from different laboratories.
methodsHere we present a digital droplet PCR (ddPCR) protocol for absolute quantification of avian haemosporidians in raptors. Novel primers were designed that target a conserved fragment of a rRNA region of the mitochondrial genome of the parasites. Sensitivity and repeatability were evaluated compared to qPCR and other assays.
resultsThis ddPCR assay enables accurate quantification of haemosporidian parasites belonging to Plasmodium, Haemoproteus and Leucocytozoon with minimum infection quantities of 10
conclusionsWe developed a novel ddPCR assay enabling accurate quantification of avian haemosporidians without golden samples or standard curves. This assay can be used as a robust method for investigating infection patterns in different host-parasite assemblages and can facilitate the comparison of results from different laboratories.
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