Evidence map›Paper›PMID 32679873›Full record

ArticleInternational journal of molecular sciences2020

Genetically Encoded Fluorescent Sensor for Poly-ADP-Ribose.

Ekaterina O Serebrovskaya, Nadezda M Podvalnaya, Varvara V Dudenkova, Anna S Efremova, Nadya G Gurskaya, Dmitry A Gorbachev, Artem V Luzhin, Omar L Kantidze, Elena V Zagaynova, Stanislav I Shram and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Article
  3. Poly-ADP-ribosylation dynamics, signaling, and analysis.Environmental and molecular mutagenesis · 2024
    Review
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 1 country.

Ekaterina O SerebrovskayaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.ORCID 0000-0002-4967-7165
Nadezda M PodvalnayaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
Varvara V DudenkovaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, Minin and Pozharsky Sq. 10/1, 603005 Nizhny Novgorod, Russia.
Anna S EfremovaInstitute of Molecular Genetics, Kurchatova Sq. 2, 123182 Moscow, Russia.
Nadya G GurskayaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.ORCID 0000-0002-8028-8176
Dmitry A GorbachevShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
Artem V LuzhinInstitute of Gene Biology, Vavilova 34/5, 119334 Moscow, Russia.ORCID 0000-0002-8859-5306
Omar L KantidzeInstitute of Gene Biology, Vavilova 34/5, 119334 Moscow, Russia.ORCID 0000-0002-7507-7307
Elena V ZagaynovaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, Minin and Pozharsky Sq. 10/1, 603005 Nizhny Novgorod, Russia.
Stanislav I ShramInstitute of Molecular Genetics, Kurchatova Sq. 2, 123182 Moscow, Russia.
Konstantin A LukyanovShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.ORCID 0000-0001-9845-2088
Institute of Bioorganic Chemistry · RUKurchatov Institute · RUInstitute of Gene Biology · RUPrivolzhsky Research Medical University · RUPirogov Russian National Research Medical University · RU

Funding

Ministry of Science and Higher Education of the Russian Federation RFMEFI62117X0018Russian Foundation for Basic Research 16-04-01870Russian Science Support Foundation 20-65-46018
6 · The paper itself

Abstract

Poly-(ADP-ribosyl)-ation (PARylation) is a reversible post-translational modification of proteins and DNA that plays an important role in various cellular processes such as DNA damage response, replication, transcription, and cell death. Here we designed a fully genetically encoded fluorescent sensor for poly-(ADP-ribose) (PAR) based on Förster resonance energy transfer (FRET). The WWE domain, which recognizes iso-ADP-ribose internal PAR-specific structural unit, was used as a PAR-targeting module. The sensor consisted of cyan Turquoise2 and yellow Venus fluorescent proteins, each in fusion with the WWE domain of RNF146 E3 ubiquitin ligase protein. This bipartite sensor named sPARroW (sensor for PAR relying on WWE) enabled monitoring of PAR accumulation and depletion in live mammalian cells in response to different stimuli, namely hydrogen peroxide treatment, UV irradiation and hyperthermia.

Indexed as

Bacterial ProteinsBiosensing TechniquesCell LineFluorescence Resonance Energy TransferFluorescent DyesHumansLuminescent ProteinsOpen Reading FramesPoly Adenosine Diphosphate RiboseProtein DomainsRecombinant Fusion ProteinsUbiquitin-Protein LigasesBacterial ProteinsFluorescent DyesLuminescent ProteinsPoly Adenosine Diphosphate RiboseRecombinant Fusion ProteinsRNF146 protein, humanUbiquitin-Protein Ligasesyellow fluorescent protein, BacteriaDNA damage resposefluorescent proteinFRETPARsensorWWE-domai

Identifiers

PMID32679873
PMCPMC7404130
OpenAlexW3043199341

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.