ArticleCurrent biology : CB2020
Comprehensive Chromosome End Remodeling during Programmed DNA Elimination.
Article in Current biology : CB, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 52 citations in OpenAlex.
- The mechanistic and evolutionary diversity of programmed DNA elimination.Nature reviews. Genetics · 2026Review
- Programmed DNA elimination was present in the last common ancestor ofbioRxiv : the preprint server for biology · 2026Article
- Genome evolution in parthenogenetic nematodes shaped by chromosome rearrangements and introgression.Genome research · 2026Article
- The tiny germline chromosomes of Paramecium aurelia have an exceptionally high recombination rate and are capped by a new class of Helitrons.BMC biology · 2026Article
- A sequence motif for DNA double-strand break and telomere healing during programmed DNA elimination.bioRxiv : the preprint server for biology · 2026Article
- Argonautes and small RNAs associated with nematode programmed DNA elimination.bioRxiv : the preprint server for biology · 2025Article
- The biological and evolutionary consequences of competition between DNA sequences that benefit the cell and DNA sequences that benefit themselves.Nucleic acids research · 2025Review
- Units containing telomeric repeats are prevalent in subtelomeric regions of a Mesorhabditis isolate collected from the Republic of Korea.Genes & genomics · 2024Article
- End resection and telomere healing of DNA double-strand breaks during nematode programmed DNA elimination.Nucleic acids research · 2024Article
- Chromosome fusion and programmed DNA elimination shape karyotypes of nematodes.Current biology : CB · 2024Article
- End resection and telomere healing of DNA double-strand breaks during nematode programmed DNA elimination.bioRxiv : the preprint server for biology · 2024Article
- Scrambling the genome in cancer: causes and consequences of complex chromosome rearrangements.Nature reviews. Genetics · 2024Review
- AdvancingPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Comparative Genomics of Sex, Chromosomes, and Sex Chromosomes in Caenorhabditis elegans and Other Nematodes.Methods in molecular biology (Clifton, N.J.) · 2024Review
- A catalogue of chromosome counts for Phylum Nematoda.Wellcome open research · 2024Article
- Getting around the roundworms: Identifying knowledge gaps and research priorities for the ascarids.Advances in parasitology · 2024Article
- Chromatin diminution as a tool to study some biological problems.Comparative cytogenetics · 2024Article
- Chromosome fusion and programmed DNA elimination shape karyotypes of parasitic nematodes.bioRxiv : the preprint server for biology · 2023Article
- Ancient diversity in host-parasite interaction genes in a model parasitic nematode.Nature communications · 2023Article
- Chromosome fusions repatterned recombination rate and facilitated reproductive isolation during Pristionchus nematode speciation.Nature ecology & evolution · 2023Article
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6 authors at 4 institutions in 1 country.
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Abstract
Germline and somatic genomes are in general the same in a multicellular organism. However, programmed DNA elimination leads to a reduced somatic genome compared to germline cells. Previous work on the parasitic nematode Ascaris demonstrated that programmed DNA elimination encompasses high-fidelity chromosomal breaks and loss of specific genome sequences including a major tandem repeat of 120 bp and ~1,000 germline-expressed genes. However, the precise chromosomal locations of these repeats, breaks regions, and eliminated genes remained unknown. We used PacBio long-read sequencing and chromosome conformation capture (Hi-C) to obtain fully assembled chromosomes of Ascaris germline and somatic genomes, enabling a complete chromosomal view of DNA elimination. We found that all 24 germline chromosomes undergo comprehensive chromosome end remodeling with DNA breaks in their subtelomeric regions and loss of distal sequences including the telomeres at both chromosome ends. All new Ascaris somatic chromosome ends are recapped by de novo telomere healing. We provide an ultrastructural analysis of Ascaris DNA elimination and show that eliminated DNA is incorporated into double membrane-bound structures, similar to micronuclei, during telophase of a DNA elimination mitosis. These micronuclei undergo dynamic changes including loss of active histone marks and localize to the cytoplasm following daughter nuclei formation and cytokinesis where they form autophagosomes. Comparative analysis of nematode chromosomes suggests that chromosome fusions occurred, forming Ascaris sex chromosomes that become independent chromosomes following DNA elimination breaks in somatic cells. These studies provide the first chromosomal view and define novel features and functions of metazoan programmed DNA elimination.
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