Evidence map›Paper›PMID 32678000›Full record

ArticleJournal of physiological anthropology2020

Development and validation of an ELISA for a biomarker of thyroid dysfunction, thyroid peroxidase autoantibodies (TPO-Ab), in dried blood spots.

Geeta N Eick, Tara J Cepon-Robins, Maureen J Devlin, Paul Kowal, Larry S Sugiyama, J Josh Snodgrass

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of physiological anthropology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. [Expression of thyroglobulin antibody and thyroid peroxidase antibody in children with immune thrombocytopenia].Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 3 countries.

Geeta N EickDepartment of Anthropology, University of Oregon, Eugene, OR, 97403, USA. geeta.eick@gmail.com.ORCID http://orcid.org/0000-0001-7512-3265
Tara J Cepon-RobinsDepartment of Anthropology, University of Colorado Springs, Colorado Springs, CO, 80918, USA.
Maureen J DevlinDepartment of Anthropology, University of Michigan, Ann Arbor, MI, USA.
Paul KowalUniversity of Newcastle Research Centre for Generational Health and Ageing, Newcastle, New South Wales, Australia.
Larry S SugiyamaDepartment of Anthropology, University of Oregon, Eugene, OR, 97403, USA.
J Josh SnodgrassDepartment of Anthropology, University of Oregon, Eugene, OR, 97403, USA.
University of Oregon · USChiang Mai University · THUniversity of Colorado Colorado Springs · USUniversity of Michigan · US

Funding

Pilot and Feasibility (P and F) ProgramP30DK089503 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI RANDY J SEELEY · 2010 to 2026
$20.3M
National Science Foundation of the USA BCS-1638786NIDDK NIH HHS P30 DK089503University of Oregon Richard A Bray Fellowship
6 · The paper itself

Abstract

backgroundThe prevalence of allergic and autoimmune conditions has been steadily increasing in wealthy nations over the past century. One hypothesis put forward to explain this is the Old Friends Hypothesis, which posits that increased hygiene, urbanization, and lifestyle changes have reduced our exposure to parasites and microbes that we co-evolved with, resulting in immune dysregulation. However, research in traditionally living populations, who are exposed to greater parasite and pathogen loads such as those encountered during our evolution, is limited, in part due to a lack of minimally invasive, field-friendly biomarkers of autoimmune disorders. We therefore developed an ELISA to assess positivity for thyroid peroxidase autoantibody (TPO-Ab), an indicator of autoimmune thyroid disease, based on dried blood spot (DBS) samples.

resultsWe used the Accubind anti-thyroid peroxidase test system to screen our validation samples comprising matched fingerprick DBS, venous DBS, and plasma samples from 182 adults. After confirming that we had TPO-Ab-positive individuals in our validation sample (n = 12), we developed an indirect ELISA to measure TPO-Ab levels from one 3-mm DBS punch. The sensitivity and specificity of our assay for DBS samples ranged from 91.7-100% and 98.2-98.8%, respectively, using a cut-off value of ≥ 26 IU/mL. Intra-assay reliability for duplicate quality control DBS punches was 5.2%, while inter-assay reliability ranged from 11.5-24.4% for high, medium, and low DBS controls. Dilutional linearity ranged from 80 to 120%, and spike and recovery experiments indicated that the DBS matrix does not interfere with the detection of TPO-Ab. TPO-Ab levels remained stable in DBS samples stored at - 28 °C or - 80 °C, but decreased over time in DBS samples kept at 22 °C or at 37 °C.

conclusionsWe developed an in-house, kit-independent indirect ELISA assay to determine individuals' TPO-Ab positivity based on dried blood spots, representing a cost-effective method with potential applications in a range of research settings.

Indexed as

AdolescentAdultAutoantibodiesAutoantigensBiomarkersDried Blood Spot TestingEnzyme-Linked Immunosorbent AssayFemaleHumansIodide PeroxidaseIron-Binding ProteinsLimit of DetectionLinear ModelsMaleMiddle AgedReproducibility of ResultsAutoantibodiesAutoantigensBiomarkersIodide PeroxidaseIron-Binding ProteinsTPO protein, humanDried blood spotsIn-house immunoassayOld Friends HypothesisThyroid peroxidase autoantibodies

Identifiers

PMID32678000
PMCPMC7364519
OpenAlexW3043340603

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.