ArticleBMC infectious diseases2020
Validation of the T86I mutation in the gyrA gene as a highly reliable real time PCR target to detect Fluoroquinolone-resistant Campylobacter jejuni.
Article in BMC infectious diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 25 citations in OpenAlex.
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- Antimicrobial Resistance and Genomic Characterization ofMicroorganisms · 2024Article
- Fluoroquinolone-resistantJAC-antimicrobial resistance · 2024Article
- The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2021-2022.EFSA journal. European Food Safety Authority · 2024Article
- Genomic diversity of Campylobacter jejuni and Campylobacter coli isolated from the Ethiopian dairy supply chain.PloS one · 2024Article
- Multiplex Real-Time PCR for the Detection of Tetracycline, Ciprofloxacin, and Erythromycin Resistance Determinants from Human and FoodborneMicroorganisms · 2023Article
- Antibiotic Resistance inAntibiotics (Basel, Switzerland) · 2023Review
- Comparative genomics of Campylobacter jejuni from clinical campylobacteriosis stool specimens.Gut pathogens · 2022Article
- Identification of Therapeutic Targets in an Emerging Gastrointestinal PathogenAntibiotics (Basel, Switzerland) · 2022Article
- Genomic adaptations of Campylobacter jejuni to long-term human colonization.Gut pathogens · 2021Article
- Genomic Analysis and Antimicrobial Resistance ofFrontiers in microbiology · 2021Article
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Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCampylobacter jejuni is a leading cause of bacterial diarrhea worldwide, and increasing rates of fluoroquinolone (FQ) resistance in C. jejuni are a major public health concern. The rapid detection and tracking of FQ resistance are critical needs in developing countries, as these antimicrobials are widely used against C. jejuni infections. Detection of point mutations at T86I in the gyrA gene by real-time polymerase chain reaction (RT-PCR) is a rapid detection tool that may improve FQ resistance tracking.
methodsC. jejuni isolates obtained from children with diarrhea in Peru were tested by RT-PCR to detect point mutations at T86I in gyrA. Further confirmation was performed by sequencing of the gyrA gene.
resultsWe detected point mutations at T86I in the gyrA gene in 100% (141/141) of C. jejuni clinical isolates that were previously confirmed as ciprofloxacin-resistant by E-test. No mutations were detected at T86I in gyrA in any ciprofloxacin-sensitive isolates.
conclusionsDetection of T86I mutations in C. jejuni is a rapid, sensitive, and specific method to identify fluoroquinolone resistance in Peru. This detection approach could be broadly employed in epidemiologic surveillance, therefore reducing time and cost in regions with limited resources.
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