Evidence map›Paper›PMID 32676707›Full record

ArticleMikrochimica acta2020

Gold nanoparticle aptamer assay for the determination of histamine in foodstuffs.

Teresa Mairal Lerga, Vasso Skouridou, M Carmen Bermudo, Abdulaziz S Bashammakh, Mohammad S El-Shahawi, Abdulrahman O Alyoubi, Ciara K O'Sullivan

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Teresa Mairal LergaInterfibio Research Group, Departament d'Enginyeria Quimica, Universitat Rovira i Virgili, Avinguda Paı̈sos Catalans 26, 43007, Tarragona, Spain. teresa.mairal@urv.cat.
Vasso SkouridouInterfibio Research Group, Departament d'Enginyeria Quimica, Universitat Rovira i Virgili, Avinguda Paı̈sos Catalans 26, 43007, Tarragona, Spain.
M Carmen BermudoInterfibio Research Group, Departament d'Enginyeria Quimica, Universitat Rovira i Virgili, Avinguda Paı̈sos Catalans 26, 43007, Tarragona, Spain.
Abdulaziz S BashammakhDepartment of Chemistry, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah, 21589, Saudi Arabia.
Mohammad S El-ShahawiDepartment of Chemistry, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah, 21589, Saudi Arabia.
Abdulrahman O AlyoubiDepartment of Chemistry, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah, 21589, Saudi Arabia.
Ciara K O'SullivanInterfibio Research Group, Departament d'Enginyeria Quimica, Universitat Rovira i Virgili, Avinguda Paı̈sos Catalans 26, 43007, Tarragona, Spain. ciara.osullivan@urv.cat.

Funding

King Abdulaziz University NA
6 · The paper itself

Abstract

The development of a gold nanoparticle aptamer assay is persued for rapid and sensitive determination of histamine in foodstuffs, which could be deployed for on-site use. The assay is based on a histamine-specific aptamer and gold nanoparticles and the salt-induced aggregation of the particles in the presence of histamine indicated by the color change from red to blue. Gold nanoparticle size, salt type, and concentration as well as aptamer concentration were optimized, and using optimum conditions, a limit of detection of 8 nM (~ 0.05 mg/kg) was obtained. Finally, the aptamer AuNP assay was applied to the determination of histamine in quality control fish samples. The histamine levels of these samples had previously been determined using HPLC and commercial ELISA kits by numerous independent laboratories and a good correlation was obtained. The developed AuNP assay is rapid, sensitive, and reproducible. Graphical abstract.

Indexed as

AnimalsAptamers, NucleotideBase SequenceBiosensing TechniquesColorimetryDNAFishesGoldHistamineImmobilized Nucleic AcidsLimit of DetectionMetal NanoparticlesReproducibility of ResultsSeafoodAptamers, NucleotideDNAGoldHistamineImmobilized Nucleic AcidsAptamerColorimetric assayFood safetyGold nanoparticlesHistamineScombroid

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.