ArticleMolecules (Basel, Switzerland)2020
Dried Urine Microsampling Coupled to Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) for the Analysis of Unconjugated Anabolic Androgenic Steroids.
Article in Molecules (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Sustainable and renewable approaches in biomaterial-based integrated dried urine sampling/solid-phase extraction devices for drugs and metabolites: challenges, bioanalytical applications, and future directions.Analytical and bioanalytical chemistry · 2025Review
- Dried Spot Paradigm: Problems and Prospects in Proteomics.International journal of molecular sciences · 2025Review
- LC-HRMS screening procedure for the detection of 11 different classes of prohibited substances in dried urine spots for doping control purposes.Analytical and bioanalytical chemistry · 2025Article
- Volumetric Absorptive Microsampling in Toxicology.Toxics · 2024Review
- Determination of clostebol residues in the urine of slaughter animals using liquid chromatography-tandem mass spectrometry.Journal of veterinary research · 2024Article
- Review
- Recent progress on the detection of animal-derived food stimulants using mass spectrometry-based techniques.Frontiers in nutrition · 2023Review
- Volumetric Absorptive Microsampling (VAMS) for Targeted LC-MS/MS Determination of Tryptophan-Related Biomarkers.Molecules (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Testing and monitoring anabolic androgenic steroids in biological fluids is a key activity in anti-doping practices. In this study, a novel approach is proposed, based on dried urine microsampling through two different workflows: dried urine spots (DUS) and volumetric absorptive microsampling (VAMS). Both techniques can overcome some common drawbacks of urine sampling, such as analyte instability and storage and transportation problems. Using an original, validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, exogenous and endogenous unconjugated steroids were analysed. Despite the limitations of microsampling volume, good sensitivity was obtained (limit of quantitation ≤1.5 ng/mL for all analytes), with satisfactory precision (relative standard deviation <7.6%) and absolute recovery (>70.3%). Both microsampling platforms provide reliable results, in good agreement with those obtained from urine.
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Registered trials
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