ArticlePLoS pathogens2020
Ancient co-option of an amino acid ABC transporter locus in Pseudomonas syringae for host signal-dependent virulence gene regulation.
Article in PLoS pathogens, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Bacterial degradation of a plant toxin and nutrient competition with commensals trade off to constrain pathogen growth.mSystems · 2026Article
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- Effect ofAmerican journal of translational research · 2026Article
- GacA uses two distinct regulatory mechanisms to control the biosynthesis of 2,4-diacetylphloroglucinol inApplied and environmental microbiology · 2025Article
- Polyyne production is regulated by the transcriptional regulators PgnC and GacA inApplied and environmental microbiology · 2025Article
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- Fostering plant protection against certain bacterial diseases through quorum-sensing signal molecules: a critical review.Frontiers in plant science · 2025Review
- Transporter-mediated depletion of extracellular proline directly contributes to plant pattern-triggered immunity against a bacterial pathogen.Nature communications · 2024Article
- A computational model of Pseudomonas syringae metabolism unveils a role for branched-chain amino acids in Arabidopsis leaf colonization.PLoS computational biology · 2023Article
- Transcription factor expression levels and environmental signals constrain transcription factor innovation.Microbiology (Reading, England) · 2023Article
- Microbial-Derived Tryptophan Metabolites and Their Role in Neurological Disease: Anthranilic Acid and Anthranilic Acid Derivatives.Microorganisms · 2023Review
- HexR Transcription Factor Contributes toMicroorganisms · 2023Article
- Type III Secretion System Repressor RhpR Induces GrlP, a Glycine-Rich Outer Membrane Lipoprotein with Functions in Regulating the Periplasmic Space and Pleiotropic Responses.Applied and environmental microbiology · 2023Article
- Article
- Two-component systems regulate bacterial virulence in response to the host gastrointestinal environment and metabolic cues.Virulence · 2022Review
- Hierarchical regulation of Burkholderia glumae type III secretion system by GluR response regulator and Lon protease.Molecular plant pathology · 2022Article
- Pseudomonas syringae pv. tomato infection of tomato plants is mediated by GABA and l-Pro chemoperception.Molecular plant pathology · 2022Article
- Maintenance of tRNA and elongation factors supports T3SS proteins translational elongations in pathogenic bacteria during nutrient starvation.Cell & bioscience · 2022Article
- Identification of Indole-3-Acetic Acid-Regulated Genes inJournal of bacteriology · 2022Article
- Pseudomonas syringae senses polyphenols via phosphorelay crosstalk to inhibit virulence.EMBO reports · 2021Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Pathogenic bacteria frequently acquire virulence traits via horizontal gene transfer, yet additional evolutionary innovations may be necessary to integrate newly acquired genes into existing regulatory pathways. The plant bacterial pathogen Pseudomonas syringae relies on a horizontally acquired type III secretion system (T3SS) to cause disease. T3SS-encoding genes are induced by plant-derived metabolites, yet how this regulation occurs, and how it evolved, is poorly understood. Here we report that the two-component system AauS-AauR and substrate-binding protein AatJ, proteins encoded by an acidic amino acid-transport (aat) and -utilization (aau) locus in P. syringae, directly regulate T3SS-encoding genes in response to host aspartate and glutamate signals. Mutants of P. syringae strain DC3000 lacking aauS, aauR or aatJ expressed lower levels of T3SS genes in response to aspartate and glutamate, and had decreased T3SS deployment and virulence during infection of Arabidopsis. We identified an AauR-binding motif (Rbm) upstream of genes encoding T3SS regulators HrpR and HrpS, and demonstrated that this Rbm is required for maximal T3SS deployment and virulence of DC3000. The Rbm upstream of hrpRS is conserved in all P. syringae strains with a canonical T3SS, suggesting AauR regulation of hrpRS is ancient. Consistent with a model of conserved function, an aauR deletion mutant of P. syringae strain B728a, a bean pathogen, had decreased T3SS expression and growth in host plants. Together, our data suggest that, upon acquisition of T3SS-encoding genes, a strain ancestral to P. syringae co-opted an existing AatJ-AauS-AauR pathway to regulate T3SS deployment in response to specific host metabolite signals.
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