ArticlePloS one2020
Use of a highly specific kinase inhibitor for rapid, simple and precise synchronization of Plasmodium falciparum and Plasmodium knowlesi asexual blood-stage parasites.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 48 citations in OpenAlex.
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- The inner membrane complex protein, IMC55, is dispensable for intraerythrocytic development ofMicrobiology spectrum · 2026Article
- Spatiotemporal dynamics of signal dependent exocytosis and parasitophorous vacuolar membrane rupture during Plasmodium falciparum egress.PLoS pathogens · 2026Article
- Accessing invasion-competent Plasmodium vivax merozoites reveals antibody-dependent NK cell activation.Research square · 2026Article
- Microfluidic platform for automatic quantification of malaria parasite invasion under physiological flow conditions.Lab on a chip · 2026Article
- Plasmodium ARK1 regulates spindle formation during atypical mitosis and forms a divergent chromosomal passenger complex.Nature communications · 2026Article
- Physiology of invasiveFrontiers in cellular and infection microbiology · 2026Review
- The inner membrane complex protein, IMC55, is dispensable for intra-erythrocytic development ofbioRxiv : the preprint server for biology · 2025Article
- Plasmodium ARK1 regulates spindle formation during atypical mitosis and forms a divergent chromosomal passenger complex.Research square · 2025Article
- GRA12 is a common virulence factor across Toxoplasma gondii strains and mouse subspecies.Nature communications · 2025Article
- Plasmodium RON11 triggers biogenesis of the merozoite rhoptry pair and is essential for erythrocyte invasion.PLoS biology · 2024Article
- Application of optical tweezer technology reveals that PfEBA and PfRH ligands, not PfMSP1, play a central role in Plasmodium falciparum merozoite-erythrocyte attachment.PLoS pathogens · 2024Article
- Drug-induced stress mediatesMicrobiology spectrum · 2024Article
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- Protein kinase PfPK2 mediated signalling is critical for host erythrocyte invasion by malaria parasite.PLoS pathogens · 2023Article
- Distribution of malaria parasite-derived phosphatidylcholine in the infected erythrocyte.mSphere · 2023Article
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Authors and funding
7 authors at 1 institution in 1 country.
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Abstract
During the course of the asexual erythrocytic stage of development, Plasmodium spp. parasites undergo a series of morphological changes and induce alterations in the host cell. At the end of this stage, the parasites egress from the infected cell, after which the progeny invade a new host cell. These processes are rapid and occur in a time-dependent manner. Of particular importance, egress and invasion of erythrocytes by the parasite are difficult to capture in an unsynchronized culture, or even a culture that has been synchronized within a window of one to several hours. Therefore, precise synchronization of parasite cultures is of paramount importance for the investigation of these processes. Here we describe a method for synchronizing Plasmodium falciparum and Plasmodium knowlesi asexual blood stage parasites with ML10, a highly specific inhibitor of the cGMP-dependent protein kinase (PKG) that arrests parasite growth approximately 15 minutes prior to egress. This inhibitor allows parasite cultures to be synchronized so that all parasites are within a window of development of several minutes, with a simple wash step. Furthermore, we show that parasites remain viable for several hours after becoming arrested by the compound and that ML10 has advantages, owing to its high specificity and low EC50, over the previously used PKG inhibitor Compound 2. Here, we demonstrate that ML10 is an invaluable tool for the study of Plasmodium spp. asexual blood stage biology and for the routine synchronization of P. falciparum and P. knowlesi cultures.
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