Evidence map›Paper›PMID 32672418›Full record

ArticleJournal of cellular and molecular medicine2020

Endoplasmic reticulum stress-induced exosomal miR-27a-3p promotes immune escape in breast cancer via regulating PD-L1 expression in macrophages.

Xiaoli Yao, Yi Tu, Yulin Xu, Yueyue Guo, Feng Yao, Xinghua Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular and molecular medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 125 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
125citing papers in PubMed, 2 pooled it
11.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

125 citing papers in PubMed, 2 syntheses or guidelines pooled it, 195 citations in OpenAlex.

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65 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Xiaoli YaoDepartment of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, China.
Yi TuDepartment of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, China.
Yulin XuDepartment of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, China.
Yueyue GuoDepartment of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, China.
Feng YaoDepartment of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, China.
Xinghua ZhangDepartment of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, China.ORCID 0000-0002-2002-1082
Wuhan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Immune escape of breast cancer cells contributes to breast cancer pathogenesis. Tumour microenvironment stresses that disrupt protein homeostasis can produce endoplasmic reticulum (ER) stress. The miRNA-mediated translational repression of mRNAs has been extensively studied in regulating immune escape and ER stress in human cancers. In this study, we identified a novel microRNA (miR)-27a-3p and investigated its mechanistic role in promoting immune evasion. The binding affinity between miR-27a-3p and MAGI2 was predicted using bioinformatic analysis and verified by dual-luciferase reporter assay. Ectopic expression and inhibition of miR-27a-3p in breast cancer cells were achieved by transduction with mimics and inhibitors. Besides, artificial modulation of MAGI2 and PTEN was done to explore their function in ER stress and immune escape of cancer cells. Of note, exosomes were derived from cancer cells and co-cultured with macrophages for mechanistic studies. The experimental data suggested that ER stress biomarkers including GRP78, PERK, ATF6, IRE1α and PD-L1 were overexpressed in breast cancer tissues relative to paracancerous tissues. Endoplasmic reticulum stress promoted exosome secretion and elevated exosomal miR-27a-3p expression. Elevation of miR-27a-3p and PD-L1 levels in macrophages was observed in response to exosomes-overexpressing miR-27a-3p in vivo and in vitro. miR-27a-3p could target and negatively regulate MAGI2, while MAGI2 down-regulated PD-L1 by up-regulating PTEN to inactivate PI3K/AKT signalling pathway. Less CD4

Indexed as

ApoptosisB7-H1 AntigenBiomarkers, TumorCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCell Line, TumorEndoplasmic Reticulum Chaperone BiPEndoplasmic Reticulum StressExosomesFemaleHumansInterleukin-2MacrophagesMCF-7 CellsMicroRNAsMiddle AgedB7-H1 AntigenBiomarkers, TumorCD274 protein, humanEndoplasmic Reticulum Chaperone BiPHSPA5 protein, humanInterleukin-2MicroRNAsMIRN27 microRNA, humanbreast cancerendoplasmic reticulumexosomesmacrophagesMicroRNA-27a-3pprogrammed cell death-Ligand 1tumour immune evasion of breast cancer cells

Identifiers

PMID32672418
PMCPMC7520328
OpenAlexW3042590929

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.