ArticleJournal of assisted reproduction and genetics2020
Culture of human ovarian tissue in xeno-free conditions using laminin components of the human ovarian extracellular matrix.
Article in Journal of assisted reproduction and genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- Bioengineering trends in female reproduction: a systematic review.Human reproduction update · 2022Pooled it
- Low LAMA2 expression may affect drug resistance and prognosis in ovarian cancer via positive LGR5 coexpression.Translational cancer research · 2026Article
- Engineering strategies and challenges in building the follicular microenvironment for a bioprosthetic ovary.Best practice & research. Clinical obstetrics & gynaecology · 2025Review
- Extracellular matrix: unlocking new avenues in cancer treatment.Biomarker research · 2025Review
- Silk-Ovarioids: establishment and characterization of a human ovarian primary cell 3D-model system.Human reproduction open · 2025Article
- The profile of steroid hormones in human fetal and adult ovaries.Reproductive biology and endocrinology : RB&E · 2024Article
- Effects of chemicalHuman reproduction open · 2024Article
- Bioengineered 3D Ovarian Models as Paramount Technology for Female Health Management and Reproduction.Bioengineering (Basel, Switzerland) · 2023Review
- Co-culture of human cryopreserved fragmented ovarian tissue with theca progenitor cells derived from theca stem cells.Journal of assisted reproduction and genetics · 2023Article
- A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells.ACS applied materials & interfaces · 2023Article
- Transcriptomic Profile of New Gene Markers Encoding Proteins Responsible for Structure of Porcine Ovarian Granulosa Cells.Biology · 2021Article
- Comprehensive Analysis of the Expression and Prognosis for Laminin Genes in Ovarian Cancer.Pathology oncology research : POR · 2021Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
purposeOur purpose was to identify human ovarian extracellular matrix (ECM) components that would support in vitro culture of human ovarian tissue and be compatible with possible future clinical applications. We characterized ovarian expression of laminins and selected three laminin tripeptides for culture experiments to be compared with Matrigel, an undefined and animal-based mixture of ECM components.
methodsExpression of the 12 laminin genes was determined on transcript and protein levels using cortical tissue samples (n = 6), commercial ovary RNA (n = 1), follicular fluid granulosa cells (n = 20), and single-cell RNA-sequencing data. Laminin 221 (LN221), LN521, LN511, and their mixture were chosen for a 7-day culture experiment along with Matrigel using tissue from 17 patients. At the end of the culture, follicles were evaluated by scoring and counting from serial tissue sections, apoptosis measured using in situ TUNEL assay, proliferation by Ki67 staining, and endocrine function by quantifying steroids in culture media using UPLC-MS/MS.
resultsApproximately half of the cells in ovarian cortex expressed at least one laminin gene. The overall most expressed laminin α-chains were LAMA2 and LAMA5, β-chains LAMB1 and LAMB2, and γ-chain LAMC1. In culture experiments, LN221 enhanced follicular survival compared with Matrigel (p < 0.001), whereas tissue cultured on LN521 had higher proportion of secondary follicles (p < 0.001). LN511 and mixture of laminins did not support the cultures leading to lower follicle densities and higher apoptosis. All cultures produced steroids and contained proliferating cells.
conclusionsLN221 and LN521 show promise in providing xeno-free growth substrates for human ovarian tissue cultures, which may help in further development of folliculogenesis in vitro for clinical practices. The system could also be used for identification of adverse effects of chemicals in ovaries.
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