ArticleFrontiers in microbiology2020
Establishing Polycistronic Expression in the Model Microorganism
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 26 citations in OpenAlex.
- 2A peptides enable simplified discovery and heterologous production of fungal bioactive metabolites in Yarrowia lipolytica.Applied microbiology and biotechnology · 2026Article
- Expression and Detection of Heterologous Proteins in Ustilago maydis.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Efficient Introduction of Genetic Elements at Specific Insertion Loci.Methods in molecular biology (Clifton, N.J.) · 2026Article
- An Efficient Colony PCR Method for Construct Verification in the Basidiomycete Ustilago maydis.Methods in molecular biology (Clifton, N.J.) · 2026Article
- In Vivo Online Monitoring of Intracellular Lipid Accumulation inEngineering in life sciences · 2025Article
- Deciphering the RNA-binding protein network during endosomal mRNA transport.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The mRNA stability factor Khd4 defines a specific mRNA regulon for membrane trafficking in the pathogenProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Metabolic Changes and Antioxidant Response inJournal of fungi (Basel, Switzerland) · 2023Article
- Engineering and Implementation of Synthetic Molecular Tools in the Basidiomycete FungusJournal of fungi (Basel, Switzerland) · 2023Article
- A MademoiseLLE domain binding platform links the key RNA transporter to endosomes.PLoS genetics · 2022Article
- Highly feasible immunoprotective multicistronic SARS-CoV-2 vaccine candidate blending novel eukaryotic expression andJournal of advanced research · 2022Article
- Tetracycline-controlled (TetON) gene expression system for the smut fungusFrontiers in fungal biology · 2022Article
- Genomic discovery and structural dissection of a novel type of polymorphic toxin system in gram-positive bacteria.Computational and structural biotechnology journal · 2022Article
- Construction of Messenger RNA (mRNA) Probes Delivered By Lipid Nanoparticles to Visualize Intracellular Protein Expression and Localization at Organelles.Advanced materials (Deerfield Beach, Fla.) · 2021Article
- A Novel Potent Carrier for Unconventional Protein Export inFrontiers in cell and developmental biology · 2021Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic microorganisms use monocistronic mRNAs to encode proteins. For synthetic biological approaches like metabolic engineering, precise co-expression of several proteins in space and time is advantageous. A straightforward approach is the application of viral 2A peptides to design synthetic polycistronic mRNAs in eukaryotes. During translation of these peptides the ribosome stalls, the peptide chain is released and the ribosome resumes translation. Thus, two independent polypeptide chains can be encoded from a single mRNA when a 2A peptide sequence is placed inbetween the two open reading frames. Here, we establish such a system in the well-studied model microorganism
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