ArticleScientific reports2020
The discovery of a new antibody for BRIL-fused GPCR structure determination.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Universal pipeline for high-resolution GPCR structure determination.Nature structural & molecular biology · 2026Article
- A structural overview of G-protein-coupled receptors in neurological disorders.Acta pharmacologica Sinica · 2026Review
- Integrated Computer-Aided Drug Design: Advances in GPCR Natural Ligand Discovery.Cell biochemistry and biophysics · 2026Review
- GPCR Heterodimers: Implications for Biased Signaling.Handbook of experimental pharmacology · 2026Review
- A fiducial-assisted strategy compatible with resolving small MFS transporter structures in multiple conformations using cryo-EM.Nature communications · 2025Article
- G Protein-Coupled Receptor-Ligand Pose and Functional Class Prediction.International journal of molecular sciences · 2024Article
- A method for structure determination of GPCRs in various states.Nature chemical biology · 2024Article
- Structural studies of serotonin receptor family.BMB reports · 2023Review
- G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.Signal transduction and targeted therapy · 2023Review
- Structural insight into an anti-BRIL Fab as a G-protein-coupled receptor crystallization chaperone.Acta crystallographica. Section D, Structural biology · 2023Article
- MUG: A mutation overview of GPCR subfamily A17 receptors.Computational and structural biotechnology journal · 2023Article
- The Angiotensin ATPharmacological reviews · 2022Review
- Evolutionary association of receptor-wide amino acids with G protein-coupling selectivity in aminergic GPCRs.Life science alliance · 2022Article
- Class A and C GPCR Dimers in Neurodegenerative Diseases.Current neuropharmacology · 2022Review
- Structural insights into ligand recognition and activation of angiotensin receptors.Trends in pharmacological sciences · 2021Review
- Moving toward generalizable NZ-1 labeling for 3D structure determination with optimized epitope-tag insertion.Acta crystallographica. Section D, Structural biology · 2021Article
- BIO-GATS: A Tool for Automated GPCR Template Selection Through a Biophysical Approach for Homology Modeling.Frontiers in molecular biosciences · 2021Article
- Structure, dynamics and lipid interactions of serotonin receptors: excitements and challenges.Biophysical reviews · 2020Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
G-protein-coupled receptors (GPCRs)-the largest family of cell-surface membrane proteins-mediate the intracellular signal transduction of many external ligands. Thus, GPCRs have become important drug targets. X-ray crystal structures of GPCRs are very useful for structure-based drug design (SBDD). Herein, we produced a new antibody (SRP2070) targeting the thermostabilised apocytochrome b562 from Escherichia coli M7W/H102I/R106L (BRIL). We found that a fragment of this antibody (SRP2070Fab) facilitated the crystallisation of the BRIL-tagged, ligand bound GPCRs, 5HT
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