ArticleProceedings of the National Academy of Sciences of the United States of America2020
Complete microviscosity maps of living plant cells and tissues with a toolbox of targeting mechanoprobes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed, 88 citations in OpenAlex.
- Neural Network-Based System for Rapid Diagnostic Decision Support Using FLIM Data: Segmentation and Classification in Liver, Skin, and Pancreatic Tissues.International journal of molecular sciences · 2026Article
- Getting nuclear size just right - emerging mechanisms regulating nuclear scaling and morphology.Journal of cell science · 2026Review
- Membrane remodelling mediates lipopeptide-induced immunity in Arabidopsis.Nature plants · 2026Article
- The mechanical properties ofScience advances · 2026Article
- The diverse roles of host membranes in plant-microbe interactions.PLoS pathogens · 2026Article
- The function of the Arabidopsis receptor kinase THESEUS1 in plant cell wall integrity maintenance: from evolutionary origin to future perspectives.The Plant journal : for cell and molecular biology · 2026Review
- Triphenylphosphonium is an effective targeting moiety for plant mitochondria.The New phytologist · 2025Article
- Long axial range 3D single-particle tracking using birefringent substrates.Nature communications · 2025Article
- Article
- Editorial: Photodynamic Therapy (3rd Edition).Biomedicines · 2025Article
- Sterols in plant biologyCell surface (Amsterdam, Netherlands) · 2025Review
- CarboTag: a modular approach for live and functional imaging of plant cell walls.Nature methods · 2025Article
- One-Step Solvothermal Synthesis of Carbon Dots for Rapid and Accurate Determination of Hemin Content.Molecules (Basel, Switzerland) · 2025Article
- The viscoelastic properties of Nicotiana tabacum BY-2 suspension cell lines adapted to high osmolarity.BMC plant biology · 2025Article
- Investigation of simple BODIPY dyes as G-quadruplex recognizing ligands.RSC advances · 2025Article
- ATG8ylation of vacuolar membrane protects plants against cell wall damage.Nature plants · 2025Article
- Do biomolecular condensates regulate the transcriptional and post-transcriptional responses of plant roots to water deficit?Quantitative plant biology · 2025Review
- Imaging Plant Lipids with Fluorescent Reporters.Plants (Basel, Switzerland) · 2024Review
- Tutorial: fluorescence lifetime microscopy of membrane mechanosensitive Flipper probes.Nature protocols · 2024Review
- Fluorescent Molecular Rotors Quantify an Adjuvant-Induced Softening of Plant Wax.Chemical & biomedical imaging · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mechanical patterns control a variety of biological processes in plants. The microviscosity of cellular structures effects the diffusion rate of molecules and organelles, thereby affecting processes such as metabolism and signaling. Spatial variations in local viscosity are also generated during fundamental events in the cell life cycle. While crucial to a complete understanding of plant mechanobiology, resolving subcellular microviscosity patterns in plants has remained an unsolved challenge. We present an imaging microviscosimetry toolbox of molecular rotors that yield complete microviscosity maps of cells and tissues, specifically targeting the cytosol, vacuole, plasma membrane, and wall of plant cells. These boron-dipyrromethene (BODIPY)-based molecular rotors are rigidochromic by means of coupling the rate of an intramolecular rotation, which depends on the mechanics of their direct surroundings, with their fluorescence lifetime. This enables the optical mapping of fluidity and porosity patterns in targeted cellular compartments. We show how apparent viscosity relates to cell function in the root, how the growth of cellular protrusions induces local tension, and how the cell wall is adapted to perform actuation surrounding leaf pores. These results pave the way to the noninvasive micromechanical mapping of complex tissues.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.