Evidence map›Paper›PMID 32669096›Full record

ArticleBMC ophthalmology2020

Role of short-wavelength blue light in the formation of cataracts and the expression of caspase-1, caspase-11, Gasdermin D in rat lens epithelial cells: insights into a novel pathogenic mmechanism of cataracts.

Yamin Wang, Min Zhang, Ying Sun, Xiaohui Wang, Zhaowei Song, Huazhang Li, Kexin Liu, Zhijian Li

RetractedOpen access · goldAbstract readRetracted Publication
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC ophthalmology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Yamin Wang *Department of Ophthalmology, the First Affiliated Hospital, Harbin Medical University, 143 Yiman Street, Nangang District, Harbin, China.
Min Zhang *Department of Ophthalmology, the First Affiliated Hospital, Harbin Medical University, 143 Yiman Street, Nangang District, Harbin, China.
Ying Sun *Department of Ophthalmology, the 2nd Hospital of Heilongjiang, Harbin, China.
Xiaohui WangDepartment of Ophthalmology, the First Affiliated Hospital, Harbin Medical University, 143 Yiman Street, Nangang District, Harbin, China.
Zhaowei SongDepartment of Ophthalmology, the First Affiliated Hospital, Harbin Medical University, 143 Yiman Street, Nangang District, Harbin, China.
Huazhang LiDepartment of Ophthalmology, the First Affiliated Hospital, Harbin Medical University, 143 Yiman Street, Nangang District, Harbin, China.
Kexin LiuDepartment of Ophthalmology, the First Affiliated Hospital, Harbin Medical University, 143 Yiman Street, Nangang District, Harbin, China.
Zhijian LiDepartment of Ophthalmology, the First Affiliated Hospital, Harbin Medical University, 143 Yiman Street, Nangang District, Harbin, China. lzj6515@sina.com.ORCID 0000-0003-0644-3497
Harbin Medical University · CNHeilongjiang Provincial Hospital · CN

Funding

National Natural Science Foundation of China 81870643
6 · The paper itself

Abstract

backgroundWith the popularity of blue-rich light-emitting diode (LED)-backlit display devices, our eyes are now exposed to more short-wavelength blue light than they were in the past. The goal of this study was to investigate the pathogenesis of cataracts after short-wavelength light exposure.

methodsSprague-Dawley (SD) rats were selected and randomly divided into a control group (10 rats each for the 4-, 8-, and 12-week groups) and an experimental group (10 rats each for the 4-, 8-, and 12-week groups). The rats in the experimental group were exposed to a short-wavelength blue LED lamp for 12 h per day. After exposure to the blue LED lamp, the rats were maintained in total darkness for 12 h, after which a 12-h light/dark cycle was resumed. The intensity of the lamp was 3000 lx. At the end of the short-wavelength blue LED lamp exposure (for 4, 8, and 12 weeks), the expression levels of caspase-1, caspase-11 and gasdermin D (GSDMD) were examined in rat lens epithelial cells (LECs) using qRT-PCR and Western blot analyses. An illuminance of 2500 lx was used to study the potential effect of blue LED light on HLE-B3 hLECs in vitro. AC-YVAD-CMK, a caspase-1 inhibitor, was used to confirm the pyroptosis of LECs by flow cytometry.

resultsAfter 6 weeks, cataracts developed in the experimental rats (4/20 eyes). The clarity of the lens gradually worsened with the duration of exposure. Twelve weeks later, all of the rat eyes had developed cataracts. The expression levels of caspase-1, caspase-11 and GSDMD at 4, 8, and 12 weeks were significantly higher in the samples from rats exposed to a short-wavelength blue LED lamp than in the samples from control rats (p<0.05). The proportions of double-positive hLECs were significantly increased in the 5-h and 10-h short-wavelength blue light exposure subgroups compared with the 5-h and 10-h caspase-1 inhibitor subgroups (p < 0.05).

conclusionThe data indicate that pyroptosis plays a key role in cataract induction after short-wavelength blue light exposure. This study might provide new insights into a novel pathogenic mechanism of cataracts.

Indexed as

CaspasesCataractAnimalsCaspase 1Epithelial CellsRatsRats, Sprague-DawleyCaspase 1CaspasesCaspase-1/11CataractGSDMDPyroptosisShort-wavelength blue light

Identifiers

PMID32669096
PMCPMC7364504
OpenAlexW3042466574

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.