Single-Molecule Localization Microscopy to Study Protein Organization in the Filamentous Fungus

Alexander W A F Reismann, Lea Atanasova, Susanne Zeilinger, Gerhard J Schütz

Open access · goldAbstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.2field-weighted citation impact, top 27% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Alexander W A F ReismannInstitute of Applied Physics, TU Wien, Getreidemarkt 9, A-1060 Vienna, Austria.

Lea AtanasovaInstitute of Applied Physics, TU Wien, Getreidemarkt 9, A-1060 Vienna, Austria.ORCID 0000-0002-1751-277X

Susanne ZeilingerDepartment of Microbiology, University of Innsbruck, Technikerstraße 25, A-6020 Innsbruck, Austria.ORCID 0000-0003-3112-0948

Gerhard J SchützInstitute of Applied Physics, TU Wien, Getreidemarkt 9, A-1060 Vienna, Austria.ORCID 0000-0003-1542-1089

TU Wien · AT

Funding

Austrian Science Fund P 30214-N36Austrian Science Fund FWF P 30214Technische Universität Wien MEIBioVienna Science and Technology Fund LS13-086, LS13-030

6 · The paper itself

Abstract

Single-molecule localization microscopy has boosted our understanding of biological samples by offering access to subdiffraction resolution using fluorescence microscopy methods. While in standard mammalian cells this approach has found wide-spread use, its application to filamentous fungi has been scarce. This is mainly due to experimental challenges that lead to high amounts of background signal because of ample autofluorescence. Here, we report the optimization of labeling, imaging and data analysis protocols to yield the first single-molecule localization microscopy images of the filamentous fungus

Indexed as

Cell WallFungal ProteinsHypocrealesMembrane ProteinsSingle Molecule ImagingTetraspaninsFungal ProteinsMembrane ProteinsTetraspaninsdSTORMfilamentous fungiSMLMsuperresolution microscopyTrichoderma

Identifiers

PMID32668792

DOI10.3390/molecules25143199

PMCPMC7396978

OpenAlexW3042066885

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.