ReviewJournal of neuroscience methods2020
Using rAAV2-retro in rhesus macaques: Promise and caveats for circuit manipulation.
Review in Journal of neuroscience methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 45 citations in OpenAlex.
- Retrograde transduction of dopaminergic cells in substantia nigra of the rhesus monkey.Scientific reports · 2026Article
- Projection-specific intersectional optogenetics for precise excitation and inhibition in the marmoset brain.Cell reports methods · 2026Article
- Enhanced reward coding and condition-independent dynamics in optogenetically identified corticostriatal neurons in monkeys.Nature communications · 2026Article
- Projection targeting with phototagging to study the structure and function of retinal ganglion cells.Cell reports methods · 2026Article
- Neuropixels reveal laminar microcircuit organization in monkey V1 in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Retrograde transduction of dopaminergic cells in substantia nigra of rhesus monkey.bioRxiv : the preprint server for biology · 2026Article
- Retrograde optogenetics reveals sensorimotor convergence within a corticotectal pathway of non-human primates.Current biology : CB · 2026Article
- Retrograde Optogenetics Reveals Functional Convergence within a Corticotectal Pathway of Non-Human Primates.bioRxiv : the preprint server for biology · 2025Article
- Neuropixels reveal structure-function relationships in monkey V1bioRxiv : the preprint server for biology · 2025Article
- Optogenetic Manipulation of Covert Attention in the Nonhuman Primate.Journal of cognitive neuroscience · 2025Article
- Stage-dependent role of interhemispheric pathway for motor recovery in primates.Nature communications · 2024Article
- Mesoscopic calcium imaging in a head-unrestrained male non-human primate using a lensless microscope.Nature communications · 2024Article
- A Novel Retrograde AAV Variant for Functional Manipulation of Cortical Projection Neurons in Mice and Monkeys.Neuroscience bulletin · 2024Article
- Development and characterization of a non-human primate model of disseminated synucleinopathy.Frontiers in neuroanatomy · 2024Article
- Connecting Circuits with Networks in Addiction Neuroscience: A Salience Network Perspective.International journal of molecular sciences · 2023Review
- Direct Comparison of Epifluorescence and Immunostaining for Assessing Viral Mediated Gene Expression in the Primate Brain.Human gene therapy · 2023Article
- Intravenous functional gene transfer throughout the brain of non-human primates using AAV.Research square · 2023Article
- Optogenetics in primate cortical networks.Frontiers in neuroanatomy · 2023Article
- Advances in AAV technology for delivering genetically encoded cargo to the nonhuman primate nervous system.Current research in neurobiology · 2023Review
- A taxonomy of the brain's white matter: twenty-one major tracts for the 21st century.Cerebral cortex (New York, N.Y. : 1991) · 2022Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundRecent genetic technologies such as opto- and chemogenetics allow for the manipulation of brain circuits with unprecedented precision. Most studies employing these techniques have been undertaken in rodents, but a more human-homologous model for studying the brain is the nonhuman primate (NHP). Optimizing viral delivery of transgenes encoding actuator proteins could revolutionize the way we study neuronal circuits in NHPs. NEW
methodrAAV2-retro, a popular new capsid variant, produces robust retrograde labeling in rodents. Whether rAAV2-retro's highly efficient retrograde transport would translate to NHPs was unknown. Here, we characterized the anatomical distribution of labeling following injections of rAAV2-retro encoding opsins or DREADDs in the cortico-basal ganglia and oculomotor circuits of rhesus macaques.
resultsrAAV2-retro injections in striatum, frontal eye field, and superior colliculus produced local labeling at injection sites and robust retrograde labeling in many afferent regions. In every case, however, a few brain regions with well-established projections to the injected structure lacked retrogradely labeled cells. We also observed robust terminal field labeling in downstream structures. COMPARISON WITH EXISTING METHOD(S): Patterns of labeling were similar to those obtained with traditional tract-tracers, except for some afferent labeling that was noticeably absent.
conclusionsrAAV2-retro promises to be useful for circuit manipulation via retrograde transduction in NHPs, but caveats were revealed by our findings. Some afferently connected regions lacked retrogradely labeled cells, showed robust axon terminal labeling, or both. This highlights the importance of anatomically characterizing rAAV2-retro's expression in target circuits in NHPs before moving to manipulation studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.