Evidence map›Paper›PMID 32668152›Full record

ArticleACS nano2020

Three-Dimensional Two-Color Dual-Particle Tracking Microscope for Monitoring DNA Conformational Changes and Nanoparticle Landings on Live Cells.

Yen-Liang Liu, Evan P Perillo, Phyllis Ang, Mirae Kim, Duc Trung Nguyen, Katherine Blocher, Yu-An Chen, Cong Liu, Ahmed M Hassan, Huong T Vu and 3 more

Abstract read
In one paragraph

Article in ACS nano, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Recent Advances in Single-Molecule Tracking and Imaging Techniques.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023
    Review
  6. Article
  7. Article
  8. Real-Time Feedback-Driven Single-Particle Tracking: A Survey and Perspective.Small (Weinheim an der Bergstrasse, Germany) · 2022
    Review
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yen-Liang LiuGraduate Institute of Biomedical Sciences, China Medical University, No.91, Hsueh-Shih Road, Taichung 40402, Taiwan.ORCID 0000-0002-1635-4335
Evan P PerilloDepartment of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street, BME Building, Austin, Texas 78712, United States.
Phyllis AngDepartment of Electrical and Computer Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.
Mirae KimDepartment of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street, BME Building, Austin, Texas 78712, United States.
Duc Trung NguyenDepartment of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street, BME Building, Austin, Texas 78712, United States.
Katherine BlocherDepartment of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street, BME Building, Austin, Texas 78712, United States.
Yu-An ChenDepartment of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street, BME Building, Austin, Texas 78712, United States.
Cong LiuDepartment of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street, BME Building, Austin, Texas 78712, United States.
Ahmed M HassanDepartment of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street, BME Building, Austin, Texas 78712, United States.
Huong T VuDepartment of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street, BME Building, Austin, Texas 78712, United States.
Yuan-I ChenDepartment of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street, BME Building, Austin, Texas 78712, United States.
Andrew K DunnDepartment of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street, BME Building, Austin, Texas 78712, United States.
Hsin-Chih YehDepartment of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street, BME Building, Austin, Texas 78712, United States.ORCID 0000-0001-6654-5626

Funding

Longitudinal Imaging of Cortical Small Vessel Network Structure with Two-Color Multiphoton Fluorescence MicroscopyR01NS108484 · NINDS · UNIVERSITY OF TEXAS AT AUSTIN · PI DUNN, ANDREW K · 2018 to 2022
$2.8M
Microscale Oxygenation Mapping During StrokeR01NS082518 · NINDS · UNIVERSITY OF TEXAS AT AUSTIN · PI DUNN, ANDREW K · 2013 to 2017
$1.7M
Turning a sequence barcode into a spectral barcode for single-cell analysis.R21GM129617 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI FINKELSTEIN, ILYA J, YEH, HSIN-CHIH · 2019 to 2020
$398k
NIGMS NIH HHS R21 GM129617NINDS NIH HHS R01 NS082518NINDS NIH HHS R01 NS108484
6 · The paper itself

Abstract

Here, we present a three-dimensional two-color dual-particle tracking (3D-2C-DPT) technique that can simultaneously localize two spectrally distinct targets in three dimensions with a time resolution down to 5 ms. The dual-targets can be tracked with separation distances from 33 to 250 nm with tracking precisions of ∼15 nm (for static targets) and ∼35 nm (for freely diffusing targets). Since each target is individually localized, a wealth of data can be extracted, such as the relative 3D position, the 2D rotation, and the separation distance between the two targets. Using this technique, we turn a double-stranded DNA (dsDNA)-linked dumbbell-like dimer into a nanoscopic optical ruler to quantify the bending dynamics of nicked or gapped dsDNA molecules in free solution by manipulating the design of dsDNA linkers (1-nick, 3-nt, 6-nt, or 9-nt single-strand gap), and the results show the increase of

Indexed as

MicroscopyNanoparticlesDiffusionDNAMotionDNA3D particle trackingantibody-receptor interactionsDNA conformation changenanoscopic optical rulerrotational movement

Identifiers

PMID32668152
PMCPMC7456512

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.