ArticlePloS one2020
Impact of processing method on donated human breast milk microRNA content.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- Non-Coding RNAs in Human Breast Milk: A Systematic Review.Frontiers in immunology · 2021Pooled it
- Investigating Dietary microRNA Stability and Function Using a Transgenic Milk Model With Unique microRNA Sequences.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Maternal Obesity and MicroRNAs in Breast Milk: Implications for Infant Developmental Programming.Obesity reviews : an official journal of the International Association for the Study of Obesity · 2026Review
- Impact of Holder Pasteurization on Extracellular Vesicles and Immunoregulatory MicroRNAs in Human Breast Milk.Journal of agricultural and food chemistry · 2026Article
- ncRNAs in breast milk of mothers with SLE: potential implications for neonatal immune development.Frontiers in immunology · 2026Review
- Milk-Derived Extracellular Vesicles and microRNAs: Potential Modulators of Intestinal Homeostasis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Review
- Human breast milk-derived exosomes and their positive role on neonatal intestinal health.Pediatric research · 2025Review
- Milk extracellular vesicles: A burgeoning new presence in nutraceuticals and drug delivery.Bioengineering & translational medicine · 2025Review
- From Pregnancy to Breastfeeding: The Role of Maternal Exercise in Preventing Childhood Obesity.Nutrients · 2025Review
- Effects of Different Processing on miRNA and Protein in Small Extracellular Vesicles of Goat Dairy Products.Nutrients · 2024Article
- Human Breast Milk Exosomes: Affecting Factors, Their Possible Health Outcomes, and Future Directions in Dietetics.Nutrients · 2024Review
- Impact of high-pressure processing on the bioactive compounds of milk - A comprehensive review.Journal of food science and technology · 2024Review
- Risk of Fat Mass- and Obesity-Associated Gene-Dependent Obesogenic Programming by Formula Feeding Compared to Breastfeeding.Nutrients · 2024Review
- Differential Digestive Stability of Food-Derived microRNAs: The Case of miR-30c-5p and miR-92a-3p in Polyfloral Honey.Current issues in molecular biology · 2024Article
- Neonatal enteroids absorb extracellular vesicles from human milk-fed infant digestive fluid.Journal of extracellular vesicles · 2024Article
- Donor human milk: the influence of processing technologies on its nutritional and microbial composition.Frontiers in nutrition · 2024Review
- New alternatives to holder pasteurization in processing donor milk in human milk banks.Frontiers in nutrition · 2024Review
- Recent insights into breast milk microRNA: their role as functional regulators.Frontiers in nutrition · 2024Review
- Homogenization and thermal processing reduce the concentration of extracellular vesicles in bovine milk.Food science & nutrition · 2024Article
- MicroRNA as a new bioactive component in breast milk.Non-coding RNA research · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pasteurization of donated human milk preserves it for storage and makes it safe for feeding, but at the expense of its composition, nutritional values and functions. Here, we aimed to investigate the impact of Holder Pasteurization (HoP) and High Pressure Processing (HPP) methods on miRNA in human milk and to evaluate impact of these changes on miRNA functions. Milk samples obtained from women in 50th day of lactation (n = 3) were subjected either to HoP, HPP or remained unpasteurized as a control. Subsequently, miRNA was isolated from whole material and exosomal fraction and sequenced with Illumina NextSeq 500. Sequencing data were processed, read counts were mapped to miRNA and analyzed both quantitatively with DESeq2 and functionally with DIANA mirPath v.3. While HPP caused statistically insignificant decrease in number of miRNA reads compared to unprocessed material, HoP led to 82-fold decrease in whole material (p = 0.0288) and 302-fold decrease in exosomes (p = 0.0021) not leaving enough reads for further analysis. Changes in composition of miRNA fraction before and after HPP indicated uneven stability of individual miRNAs under high pressure conditions, with miR-30d-5p identified as relatively stable and miR-29 family as sensitive to HPP. Interestingly, about 2/3 of unprocessed milk miRNA content consists of only 10 distinct miRNAs with miR-148a-3p at the top. Functional analysis of most abundant human milk miRNAs showed their involvement in signaling pathways, cell communication, proliferation and metabolism that are obviously important in rapidly growing infants. Functions of miRNAs which suffered the greatest depletion during HPP were similar to roles of the majority of unprocessed human milk's miRNA, which indicates that those functions may be weakened although not completely lost. Our findings indicate that HPP is less detrimental to human milk miRNAs than HoP and should be considered in further research on recommended processing procedures for human milk banks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.