ArticleChembiochem : a European journal of chemical biology2020
Self-Emergent Protocells Generated in an Aqueous Solution with Binary Macromolecules through Liquid-Liquid Phase Separation.
Article in Chembiochem : a European journal of chemical biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- Repair and misrepair of telomeric DNA in dynamic interactions with PML nuclear bodies and lamin B1 in doxorubicin-treated cancer cells.Nucleus (Austin, Tex.) · 2026Article
- Relationship Between Filament-Polymerizing Muscle Myosin and Droplets Generated by Liquid-Liquid Phase Separation.Chembiochem : a European journal of chemical biology · 2026Article
- A Facile Platform for One-Step Generation of Uniform Microdroplets through Dehydration-Driven Phase Separation in Microfluidics.Small methods · 2026Article
- Spontaneous Fusion of Aqueous Two-Phase Droplets: Effect of Coexisting Phospholipids on Stability.Chembiochem : a European journal of chemical biology · 2025Article
- Stabilizing Water-in-Water Emulsions Using Oil Droplets.Molecules (Basel, Switzerland) · 2025Article
- Rapid and High-Yielding Purification of DNA Self-Assembled Structures by Aqueous Two-Phase System.Methods in molecular biology (Clifton, N.J.) · 2025Article
- DNA-empowered synthetic cells as minimalistic life forms.Nature reviews. Chemistry · 2024Review
- Membranous and Membraneless Interfaces-Origins of Artificial Cellular Complexity.Life (Basel, Switzerland) · 2023Article
- Spatial reorganization of analytes in charged aqueous microdroplets.Chemical science · 2022Article
- Water-in-Water Droplets Selectively Uptake Self-Assembled DNA Nano/Microstructures: a Versatile Method for Purification in DNA Nanotechnology.Chembiochem : a European journal of chemical biology · 2022Article
- Building programmable multicompartment artificial cells incorporating remotely activated protein channels using microfluidics and acoustic levitation.Nature communications · 2022Article
- Recent advances in surface modification of micro- and nano-scale biomaterials with biological membranes and biomolecules.Frontiers in bioengineering and biotechnology · 2022Review
- Emergence of uniform linearly-arranged micro-droplets entrapping DNA and living cells through water/water phase-separation.Scientific reports · 2021Article
- Proliferating coacervate droplets as the missing link between chemistry and biology in the origins of life.Nature communications · 2021Article
- Primitive Compartmentalization for the Sustainable Replication of Genetic Molecules.Life (Basel, Switzerland) · 2021Review
- Aqueous Triple-Phase System in Microwell Array for Generating Uniform-Sized DNA Hydrogel Particles.Frontiers in genetics · 2021Article
- Self-Emergent Protocells Generated in an Aqueous Solution with Binary Macromolecules through Liquid-Liquid Phase Separation.Chembiochem : a European journal of chemical biology · 2020Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently, liquid-liquid phase separation (LLPS) has attracted considerable attention among researchers in the life sciences as a plausible mechanism for the generation of microstructures inside cells. LLPS occurs through multiple nonspecific interactions and does not always require a lock-and-key interaction with a binary macromolecular solution. The remarkable features of LLPS include the non-uniform localization and concentration of solutes, resulting in the ability to isolate certain chemical systems and thereby parallelize multiple chemical reactions within the limited space of a living cell. We report that, by using the macromolecules, poly(ethylene glycol) (PEG) and dextran, that exhibit LLPS in an aqueous solution, cell-sized liposomes are spontaneously formed therein in the presence of phospholipids. In this system, LLPS is generated through the depletion effect of macromolecules. The results showed that cell-like microdroplets entrapping DNA wrapped by a phospholipid layer emerge in a self-organized manner.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.