ArticleRevista do Instituto de Medicina Tropical de Sao Paulo2020
Culture isolation and molecular identification of Blastocystis sp. in Brazilian human isolates: preliminary results.
Article in Revista do Instituto de Medicina Tropical de Sao Paulo, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed, 10 citations in OpenAlex.
- From farm to flask: stable genotypes and shifting microbiomes in the ecological dynamics of Balantioides coli from a One Health perspective.Parasites & vectors · 2026Article
- Prevalence and Genetic Diversity of Blastocystis sp. in Humans, Dogs and Cats in Gabon: A One Health Perspective.Zoonoses and public health · 2026Article
- Prevalence of Blastocystis sp. in Morocco: Comparative assessment of three diagnostic methods and characterization of parasite forms in Jones' culture medium.Parasite (Paris, France) · 2023Article
- An update on the distribution ofHeliyon · 2022Article
- Next-generation sequencing reveals wide genetic diversity of Blastocystis subtypes in chickens including potentially zoonotic subtypes.Parasitology research · 2021Article
- Molecular Identification, Subtypes Distribution, and Alleles Discrimination ofIranian journal of parasitologyArticle
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Authors and funding
8 authors at 1 institution in 1 country.
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Abstract
Blastocystis sp. is a protist commonly found in stool samples of humans and animals. Biological and genetic factors of this organism remain controversial. The present study aimed to develop and implement the Blastocystis in vitro culture of Brazilian human isolates for routine use. The fecal isolates (n = 20) were maintained in our laboratory by several passages in Pavlova's medium. Cultures were monitored every 72 h by light microscopy. Genomic DNA was extracted to identify the subtypes (STs). In most isolates, the vacuolar form was prevalent. The amoeboid, granular and cystic forms were observed during in vitro cultivation. STs 1, 2, 3, 4 and 7 were identified. Our preliminary results show the generation time and forms present in the in vitro culture of Blastocystis subtypes isolated from Brazilian human isolates. Therefore, we emphasize the use of in vitro culture as a tool in future studies for the better understanding of the biological aspects of Blastocystis sp.
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