Evidence map›Paper›PMID 32666704›Full record

ArticleJournal of cellular and molecular medicine2020

Extracellular vesicle-enclosed miR-486-5p mediates wound healing with adipose-derived stem cells by promoting angiogenesis.

Yingjie Lu, Huicai Wen, Jinjun Huang, Peng Liao, Huaiwei Liao, Jun Tu, Yuanlin Zeng

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular and molecular medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
52citing papers in PubMed, 4 pooled it
5.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

52 citing papers in PubMed, 4 syntheses or guidelines pooled it, 97 citations in OpenAlex.

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  16. Role of exosome-derived miRNAs in diabetic wound angiogenesis.Molecular and cellular biochemistry · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Yingjie LuDepartment of Plastic Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, China.
Huicai WenDepartment of Plastic Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, China.
Jinjun HuangDepartment of Plastic Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, China.
Peng LiaoDepartment of Integrated Chinese and Western Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, China.
Huaiwei LiaoDepartment of Plastic Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, China.
Jun TuDepartment of Plastic Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, China.
Yuanlin ZengDepartment of Burn Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, China.ORCID 0000-0002-1096-0203
Nanchang University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Adipose-derived stem cells (ASC) are said to have a pivotal role in wound healing. Specifically, ASC-secreted extracellular vesicles (EV) carry diverse cargos such as microRNAs (miRNAs) to participate in the ASC-based therapies. Considering its effects, we aimed to investigate the role of ASC-EVs in the cutaneous wound healing accompanied with the study on the specific cargo-medicated effects on wound healing. Two full-thickness excisional skin wounds were created on mouse dorsum, and wound healing was recorded at the indicated time points followed by histological analysis and immunofluorescence staining for CD31 and α-SMA. Human skin fibroblasts (HSFs) and human microvascular endothelial cells (HMECs) were co-cultured with EVs isolated from ASC (ASC-EVs), respectively, followed by the evaluation of their viability and mobility using CCK-8, scratch test and transwell migration assays. Matrigel-based angiogenesis assays were performed to evaluate vessel-like tube formation by HMECs in vitro. ASC-EVs accelerated the healing of full-thickness skin wounds, increased re-epithelialization and reduced scar thickness whilst enhanced collagen synthesis and angiogenesis in murine models. However, miR-486-5p antagomir abrogated the ASC-EVs-induced effects. Intriguingly, miR-486-5p was found to be highly enriched in ASC-EVs, exhibiting an increase in viability and mobility of HSFs and HMECs and enhanced the angiogenic activities of HMECs. Notably, we also demonstrated that ASC-EVs-secreted miR-486-5p achieved the aforesaid effects through its target gene Sp5. Hence, our results suggest that miR-486-5p released by ASC-EVs could be a critical mediator to develop an ASC-based therapeutic strategy for wound healing.

Indexed as

AdipocytesAdipose TissueAdultAgedCells, CulturedCicatrixEndothelial CellsExtracellular VesiclesFemaleFibroblastsHumansMicroRNAsMiddle AgedNeovascularization, PathologicSkinStem CellsMicroRNAsMIRN486 microRNA, humanadipose-derived stem cellsangiogenesiscutaneous woundextracellular vesiclesmicroRNA-486-5p

Identifiers

PMID32666704
PMCPMC7520275
OpenAlexW3043385276

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.