Evidence map›Paper›PMID 32666133›Full record

ArticleEuropean biophysics journal : EBJ2020

Fast and synchronized fluctuations of cortical actin negatively correlate with nucleoli liquid-liquid phase separation in T cells.

Ishay Wohl, Oren Yakovian, Yair Razvag, Meital Reches, Eilon Sherman

Abstract read
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In one paragraph

Article in European biophysics journal : EBJ, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Spectral Analysis of ATP-Dependent Mechanical Vibrations in T Cells.Frontiers in cell and developmental biology · 2021
    Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Ishay WohlRacah Institute of Physics, The Hebrew University, 9190401, Jerusalem, Israel.
Oren YakovianRacah Institute of Physics, The Hebrew University, 9190401, Jerusalem, Israel.
Yair RazvagRacah Institute of Physics, The Hebrew University, 9190401, Jerusalem, Israel.
Meital RechesInstitute of Chemistry, The Hebrew University, 9190401, Jerusalem, Israel.
Eilon ShermanRacah Institute of Physics, The Hebrew University, 9190401, Jerusalem, Israel. eilon.sherman@mail.huji.ac.il.
Hebrew University of Jerusalem · IL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Liquid-liquid phase separation is an important mechanism by which eukaryotic cells functionally organize their intracellular content and has been related to cell malignancy and neurodegenerative diseases. These cells also undergo ATP-driven mechanical fluctuations, yet the effect of these fluctuations on the liquid-liquid phase separation remains poorly understood. Here, we employ high-resolution microscopy and atomic force microscopy of live Jurkat T cells to characterize the spectrum of their mechanical fluctuations, and to relate these fluctuations to the extent of nucleoli liquid-liquid phase separation (LLPS). We find distinct fluctuation of the cytoskeleton and of the cell diameter around 110 Hz, which depend on ATP and on myosin activity. Importantly, these fluctuations negatively correlate to nucleoli LLPS. According to a model of cell viscoelasticity, we propose that these fluctuations generate mechanical work that increases intracellular homogeneity by inhibiting LLPS. Thus, active mechanical fluctuations serve as an intracellular regulatory mechanism that could affect multiple pathophysiological conditions.

Indexed as

ActinsAdenosine TriphosphateCell NucleolusCell SeparationHumansJurkat CellsMicroscopy, Atomic ForceTime FactorsT-LymphocytesActinsAdenosine TriphosphateCortical actinDFT analysisFluctuationLiqud–liquid phase separationMyosinNucleoliPlasma membraneTension

Identifiers

PMID32666133
OpenAlexW3042672864

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.