Evidence map›Paper›PMID 32666015›Full record

ArticleAPL bioengineering2020

A novel method to make viscoelastic polyacrylamide gels for cell culture and traction force microscopy.

Elisabeth E Charrier, Katarzyna Pogoda, Robin Li, Chan Young Park, Jeffrey J Fredberg, Paul A Janmey

Abstract read
In one paragraph

Article in APL bioengineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed.

  1. Traction Force Microscopy for Viscoelastic Substrates: A Semi-Analytical Method.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  2. Article
  3. Article
  4. Article
  5. Inhibition of the MRTF-A/SRF signaling axis alleviates vocal fold scarring.Matrix biology : journal of the International Society for Matrix Biology · 2025
    Article
  6. Article
  7. Review
  8. Substrate stress relaxation regulates neural stem cell fate commitment.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  9. Mechanobiological Modulation ofACS biomaterials science & engineering · 2024
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Gradual Stress-Relaxation of Hydrogel Regulates Cell Spreading.International journal of molecular sciences · 2022
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Elisabeth E Charrier
Robin LiInstitute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Chan Young ParkDepartment of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, Massachusetts 02115, USA.
Jeffrey J FredbergDepartment of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, Massachusetts 02115, USA.

Funding

Training Program in Molecular and Integrative Physiological SciencesT32HL007118 · NHLBI · HARVARD UNIVERSITY (SCH OF PUBLIC HLTH) · PI LU, QUAN · 1985 to 2025
$14.3M
Pathological consequences of altered tissue mechanics in fibrosisR01EB017753 · NIBIB · UNIVERSITY OF PENNSYLVANIA · PI JANMEY, PAUL A, SHENOY, VIVEK · 2014 to 2025
$6.3M
Physics of bronchial epithelial unjammingR01HL148152 · NHLBI · HARVARD SCHOOL OF PUBLIC HEALTH · PI FREDBERG, JEFFREY J, PARK, JIN-AH · 2019 to 2022
$2.2M
NHLBI NIH HHS R01 HL148152NHLBI NIH HHS T32 HL007118NIBIB NIH HHS R01 EB017753
6 · The paper itself

Abstract

Polyacrylamide hydrogels are commonly used in cell biology, notably to cultivate cells on soft surfaces. Polyacrylamide gels are purely elastic and well adapted to cell culture as they are inert and can be conjugated with adhesion proteins. Here, we report a method to make viscoelastic polyacrylamide gels with mechanical properties more closely resembling biological tissues and suitable for cell culture

Identifiers

PMID32666015
PMCPMC7334032

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.