ArticleHeliyon2020
A one-step, one-tube real-time RT-PCR based assay with an automated analysis for detection of SARS-CoV-2.
Article in Heliyon, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Digital Melting Curve Analysis for Multiplex Quantification of Nucleic Acids on Droplet Digital PCR.Biosensors · 2025Article
- Diagnostics and analysis of SARS-CoV-2: current status, recent advances, challenges and perspectives.Chemical science · 2023Review
- Review
- Review of COVID-19 testing and diagnostic methods.Talanta · 2022Review
- Duplex One-Step RT-qPCR Assays for Simultaneous Detection of Genomic and Subgenomic RNAs of SARS-CoV-2 Variants.Viruses · 2022Article
- Development and validation of cost-effective one-step multiplex RT-PCR assay for detecting the SARS-CoV-2 infection using SYBR Green melting curve analysis.Scientific reports · 2022Article
- Isothermal amplification-based assays for rapid and sensitive detection of severe acute respiratory syndrome coronavirus 2: Opportunities and recent developments.Reviews in medical virology · 2022Review
- Diagnostic techniques for COVID-19: A mini-review.Journal of virological methods · 2022Review
- Melting Curve-based Assay as an Alternative Technique for the Accurate Detection of SARS-CoV-2.Advanced biomedical research · 2022Article
- Molecular Epidemiology of SARS-CoV-2 in Diverse Environmental Samples Globally.Microorganisms · 2021Article
- Validation of real-time RT-PCR for detection of SARS-CoV-2 in the early stages of the COVID-19 outbreak in the Republic of Korea.Scientific reports · 2021Article
- Performance characteristics of the Abbott Alinity m SARS-CoV-2 assay.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2021Article
- Nucleic Acid Testing of SARS-CoV-2.International journal of molecular sciences · 2021Review
- A cost-effective plan for global testing - an infection rate stratified, algorithm guided, multiple-level, continuously pooled testing strategy.The Science of the total environment · 2021Article
- Biological characteristics and biomarkers of novel SARS-CoV-2 facilitated rapid development and implementation of diagnostic tools and surveillance measures.Biosensors & bioelectronics · 2021Review
- COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR-Facts and Fallacies.International journal of molecular sciences · 2021Article
- SARS-CoV, MERS-CoV and SARS-CoV-2: A Diagnostic Challenge.Measurement : journal of the International Measurement Confederation · 2021Article
- Smart materials-integrated sensor technologies for COVID-19 diagnosis.Emergent materials · 2021Review
- Novel coronavirus disease (COVID-19) pandemic: A recent mini review.Computational and structural biotechnology journal · 2021Review
- Next-generation computational tools and resources for coronavirus research: From detection to vaccine discovery.Computers in biology and medicine · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Early diagnosis of SARS-CoV-2 infected patients is essential to control the dynamics of the COVID-19 pandemic. We develop a rapid and accurate one-step multiplex TaqMan probe-based real-time RT-PCR assay, along with a computational tool to systematically analyse the data. Our assay could detect to a limit of 15 copies of SARS-CoV-2 transcripts-based on experiments performed by spiking total human RNA with
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.