Evidence map›Paper›PMID 32665976›Full record

ArticleBiology methods & protocols2020

Optimization of Chelex 100 resin-based extraction of genomic DNA from dried blood spots.

Neta Simon, Jaclyn Shallat, Corey Williams Wietzikoski, Whitney E Harrington

Open access · goldAbstract read
In one paragraph

Article in Biology methods & protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
7.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 50 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Genetic metrics decodeFrontiers in genetics · 2026
    Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Identification and Antimicrobial Potential of Marine Sponges (International journal of microbiology · 2025
    Article
  14. UndetectedMalariaWorld journal · 2025
    Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Neta SimonDepartment of Microbiology, University of Washington, Seattle, WA, USA.
Jaclyn ShallatDepartment of Microbiology, University of Washington, Seattle, WA, USA.
Corey Williams WietzikoskiSeattle Children's Research Institute, Seattle, WA, USA.
Whitney E HarringtonSeattle Children's Research Institute, Seattle, WA, USA.ORCID 0000-0002-0121-978X
University of Washington · US

Funding

The Effect of Maternal Cells on Infant ImmunityK08AI135072 · NIAID · SEATTLE CHILDREN'S HOSPITAL · PI HARRINGTON, WHITNEY ELIZABETH · 2018 to 2022
$854k
NIAID NIH HHS K08 AI135072
6 · The paper itself

Abstract

Dried blood spots (DBS) are widely utilized as part of universal newborn screening and as a means of transporting samples from field sites. We use DBS from African field sites to assess for rare maternal-fetal cell exchange during pregnancy known as microchimerism. We aimed to develop a protocol to maximize the quantity of high-quality genomic DNA (gDNA) extracted from DBS. The total gDNA yield obtained from control DBS utilizing a Qiagen-based protocol and a Chelex

Identifiers

PMID32665976
PMCPMC7334876
OpenAlexW3022655241

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.