ArticleScientific reports2020
Multiple cryoinjuries modulate the efficiency of zebrafish heart regeneration.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Regeneration of the lizard heart after cryoinjury.Experimental physiology · 2026Article
- Review
- Heart ventricle regeneration in the lizard Eublepharis macularius, the leopard gecko.NPJ Regenerative medicine · 2026Article
- Article
- Oxidative phosphorylation is required for cardiomyocyte re-differentiation and long-term fish heart regeneration.Nature cardiovascular research · 2025Article
- Antigen presentation plays positive roles in the regenerative response to cardiac injury in zebrafish.Nature communications · 2024Article
- Zebrafish as a Model for Cardiovascular and Metabolic Disease: The Future of Precision Medicine.Biomedicines · 2024Review
- Skeletal muscle regeneration after extensive cryoinjury of caudal myomeres in adult zebrafish.NPJ Regenerative medicine · 2024Article
- Foxm1 regulates cardiomyocyte proliferation in adult zebrafish after cardiac injury.Development (Cambridge, England) · 2023Article
- The regeneration-responsive elementFrontiers in molecular neuroscience · 2023Article
- Collagen XII mediated cellular and extracellular mechanisms in development, regeneration, and disease.Frontiers in cell and developmental biology · 2023Review
- Wound Healing Insights from Flies and Fish.Cold Spring Harbor perspectives in biology · 2022Review
- Hippo-Yap/Taz signalling in zebrafish regeneration.NPJ Regenerative medicine · 2022Review
- Finding Solutions for Fibrosis: Understanding the Innate Mechanisms Used by Super-Regenerator Vertebrates to Combat Scarring.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Using Zebrafish as a Disease Model to Study Fibrotic Disease.International journal of molecular sciences · 2021Review
- Persistent Ventricle Partitioning in the Adult Zebrafish Heart.Journal of cardiovascular development and disease · 2021Article
- Zebrafish cardiac regeneration-looking beyond cardiomyocytes to a complex microenvironment.Histochemistry and cell biology · 2020Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Zebrafish can regenerate their damaged hearts throughout their lifespan. It is, however, unknown, whether regeneration remains effective when challenged with successive cycles of cardiac damage in the same animals. Here, we assessed ventricular restoration after two, three and six cryoinjuries interspaced by recovery periods. Using transgenic cell-lineage tracing analysis, we demonstrated that the second cryoinjury damages the regenerated area from the preceding injury, validating the experimental approach. We identified that after multiple cryoinjuries, all hearts regrow a thickened myocardium, similarly to hearts after one cryoinjury. However, the efficiency of scar resorption decreased with the number of repeated cryoinjuries. After six cryoinjuries, all examined hearts failed to completely resolve the fibrotic tissue, demonstrating reduced myocardial restoration. This phenotype was associated with enhanced recruitment of neutrophils and decreased cardiomyocyte proliferation and dedifferentiation at the early regenerative phase. Furthermore, we found that each repeated cryoinjury increased the accumulation of collagen at the injury site. Our analysis demonstrates that the cardiac regenerative program can be successfully activated many times, despite a persisting scar in the wounded area. This finding provides a new perspective for regenerative therapies, aiming in stimulation of organ regeneration in the presence of fibrotic tissue in mammalian models and humans.
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Registered trials
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