ArticleScientific reports2020
Optimization of sperm RNA processing for developmental research.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- On the state of chromatin in mammalian sperm.bioRxiv : the preprint server for biology · 2026Article
- Spatiotemporal translation of sperm acrosome associated proteins during early capacitation modulates sperm fertilizing ability.Journal of advanced research · 2026Article
- Effects of the Freezing Diluent Tonicity and Prefreezing Cooling Rates on Post-Thaw Sperm Quality Markers in the Pure-Bred Arabian Stallion.Veterinary medicine international · 2026Article
- The use of environmental RNA for inferring fish spawning behavior.Scientific reports · 2025Article
- Season affects sperm quality, seminal extracellular vesicles, and antioxidant-related genes in Egyptian Buffalo bulls.Scientific reports · 2025Article
- Review
- Detection and Quantification of mRNA in Mammalian Spermatozoa.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Integration of omics studies indicates that species-dependent molecular mechanisms govern male fertility.Journal of animal science and biotechnology · 2023Article
- Development of a biomolecular approach to identify sperm functions and fertility using sperm RNAs.Frontiers in cell and developmental biology · 2023Article
- Mating modifies the expression of crucial oxidative-reductive transcripts in the pig oviductal sperm reservoir: is the female ensuring sperm survival?Frontiers in endocrinology · 2023Article
- Sperm Transcriptome Analysis Accurately Reveals Male Fertility Potential in Livestock.Animals : an open access journal from MDPI · 2022Review
- Establishment of a male fertility prediction model with sperm RNA markers in pigs as a translational animal model.Journal of animal science and biotechnology · 2022Article
- Heat shock protein family D member 1 in boar spermatozoa is strongly related to the litter size of inseminated sows.Journal of animal science and biotechnology · 2022Article
- Urinary extracellular vesicles: Assessment of pre-analytical variables and development of a quality control with focus on transcriptomic biomarker research.Journal of extracellular vesicles · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent studies have demonstrated the significance of sperm RNA function as a transporter of important information directing the course of life. To determine the message contained in sperm RNA, it is necessary to optimize transcriptomic research tools. The current study was performed to optimize the processing of sperm RNA from sample storage to quantitative real-time PCR and assess the corresponding method with to evaluate male fertility and its representative markers, equatorin (EQTN) and peroxiredoxin (PRDX). Following successive steps of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments guidelines, several options were compared using boar spermatozoa. To evaluate the optimized procedures, the relationship between mRNA expression of EQTN and PRDX in spermatozoa and the fertility (litter size) of 20 individual boars was assessed. Unexpectedly, DNase treatment during RNA isolation had the deleterious effect by decreasing the RNA concentration by 56% and eliminating the correlation between EQTN and PRDX4 mRNA expression and male fertility. Moreover, when sperm RNA was processed using the corresponding method, the results showed the highest exon sequence expression, male fertility prediction power, and consistency. This optimized protocol for predicting male fertility can be used to study the transport of messages directing the life course from spermatozoon to offspring.
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