ArticleAngewandte Chemie (International ed. in English)2020
In Vitro Reconstitution Reveals a Central Role for the N-Oxygenase PvfB in (Dihydro)pyrazine-N-oxide and Valdiazen Biosynthesis.
Article in Angewandte Chemie (International ed. in English), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 44 citations in OpenAlex.
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- Synthetic pathways for microbial biosynthesis of valuable pyrazine derivatives using genetically modifiedMetabolic engineering communications · 2025Article
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- Flavour chemistry and metabolic engineering of microbial synthetic pyrazines: decoding the path to green manufacturing of food aroma components.Current research in food science · 2025Review
- Functional biosynthetic stereodivergence in a gene cluster via a dihydrosydnone N-oxide.Communications chemistry · 2024Article
- Characterization of an efficient N-oxygenase from Saccharothrix sp. and its application in the synthesis of azomycin.Biotechnology for biofuels and bioproducts · 2023Article
- Pseudomonas virulence factor controls expression of virulence genes in Pseudomonas entomophila.PloS one · 2023Article
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- Enzymatic Synthesis of Diverse Heterocycles by a Noncanonical Nonribosomal Peptide Synthetase.ACS chemical biology · 2021Article
- An efficient and regioselective biocatalytic synthesis of aromatic N-oxides by using a soluble di-iron monooxygenase PmlABCDEF produced in the Pseudomonas species.Microbial biotechnology · 2021Article
- Glycine-derived nitronates bifurcate to O-methylation or denitrification in bacteria.Nature chemistry · 2021Article
- Regulation of Plant Mineral Nutrition by Signal Molecules.Microorganisms · 2021Review
- Pseudomonas Virulence Factor Pathway Synthesizes Autoinducers That Regulate the Secretome of a Pathogen.ACS chemical biology · 2021Article
- Discovery and biosynthesis of guanipiperazine from a NRPS-like pathway.Chemical science · 2021Article
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2 authors at 1 institution in 1 country.
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Abstract
The Pseudomonas virulence factor (pvf) operon is essential for the biosynthesis of two very different natural product scaffolds: the (dihydro)pyrazine-N-oxides and the diazeniumdiolate, valdiazen. PvfB is a member of the non-heme diiron N-oxygenase enzyme family that commonly convert anilines to their nitroaromatic counterparts. In contrast, we show that PvfB catalyzes N-oxygenation of the α-amine of valine, first to the hydroxylamine and then the nitroso, while linked to the carrier protein of PvfC. PvfB modification of PvfC-tethered valine was observed directly by protein NMR spectroscopy, establishing the intermediacy of the hydroxylamine. This work reveals a central role for PvfB in the biosynthesis of (dihydro)pyrazine-N-oxides and valdiazen.
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