Evidence map›Paper›PMID 32662768›Full record

ArticleeLife2020

A two-lane mechanism for selective biological ammonium transport.

Gordon Williamson, Giulia Tamburrino, Adriana Bizior, Mélanie Boeckstaens, Gaëtan Dias Mirandela, Marcus G Bage, Andrei Pisliakov, Callum M Ives, Eilidh Terras, Paul A Hoskisson and 3 more

Erratum issuedOpen access · goldAbstract read
In one paragraph

Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
4.9field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 31 citations in OpenAlex.

  1. Article
  2. Article
  3. Chloride Gradient Is Involved in Ammonium Influx in Human Erythrocytes.International journal of molecular sciences · 2024
    Article
  4. Article
  5. Review
  6. Review
  7. Article
  8. Article
  9. Article
  10. A diazotrophy-ammoniotrophy dual growth model for the sulfate reducing bacteriumComputational and structural biotechnology journal · 2023
    Article
  11. Article
  12. Article
  13. Article
  14. Review
  15. Article
  16. Article
  17. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 2 countries.

Gordon Williamson *Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom.ORCID https://orcid.org/0000-0003-3053-8322
Giulia Tamburrino *Computational Biology, School of Life Sciences, University of Dundee, Dundee, United Kingdom.
Adriana Bizior *Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom.
Mélanie Boeckstaens *Biology of Membrane Transport Laboratory, Department of Molecular Biology, Université Libre de Bruxelles, Gosselies, Belgium.ORCID https://orcid.org/0000-0003-1629-7403
Gaëtan Dias MirandelaStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom.ORCID https://orcid.org/0000-0001-5871-6288
Marcus G BageComputational Biology, School of Life Sciences, University of Dundee, Dundee, United Kingdom.
Andrei PisliakovComputational Biology, School of Life Sciences, University of Dundee, Dundee, United Kingdom.ORCID https://orcid.org/0000-0003-1536-0589
Callum M IvesComputational Biology, School of Life Sciences, University of Dundee, Dundee, United Kingdom.ORCID http://orcid.org/0000-0003-0511-1220
Eilidh TerrasStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom.
Paul A HoskissonStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom.
Anna Maria MariniBiology of Membrane Transport Laboratory, Department of Molecular Biology, Université Libre de Bruxelles, Gosselies, Belgium.
Ulrich ZachariaeComputational Biology, School of Life Sciences, University of Dundee, Dundee, United Kingdom.
Arnaud JavelleStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom.ORCID https://orcid.org/0000-0002-3611-5737
University of Strathclyde · GBUniversity of Dundee · GBUniversité Libre de Bruxelles · BE

Funding

Fonds De La Recherche Scientifique - FNRS WELBIO grant ref: CR-2019A-05R.Natural Environment Research Council NE/M001415/1Tenovus S17-07
6 · The paper itself

Abstract

The transport of charged molecules across biological membranes faces the dual problem of accommodating charges in a highly hydrophobic environment while maintaining selective substrate translocation. This has been the subject of a particular controversy for the exchange of ammonium across cellular membranes, an essential process in all domains of life. Ammonium transport is mediated by the ubiquitous Amt/Mep/Rh transporters that includes the human Rhesus factors. Here, using a combination of electrophysiology, yeast functional complementation and extended molecular dynamics simulations, we reveal a unique two-lane pathway for electrogenic NH

Indexed as

Ion TransportAmmoniaAmmonium CompoundsEscherichia coliNitrosomonas europaeaAmmoniaAmmonium Compoundsammonium transporterbiochemistrychemical biologyE. colinitrosomonas europaearhesus proteinSaccharomyces cerevisiaeSSMEtransport selectivity

Identifiers

PMID32662768
PMCPMC7447429
OpenAlexW3043088056

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.