Evidence map›Paper›PMID 32661768›Full record

ArticleTranslational stroke research2021

Optogenetic Stimulation Reduces Neuronal Nitric Oxide Synthase Expression After Stroke.

Arjun V Pendharkar, Daniel Smerin, Lorenzo Gonzalez, Eric H Wang, Sabrina Levy, Stephanie Wang, Shunsuke Ishizaka, Masaki Ito, Haruto Uchino, Terrance Chiang and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational stroke research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.1field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 15 citations in OpenAlex.

  1. [Research progress on the neuromodulation targets in stroke rehabilitation].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Arjun V PendharkarDepartment of Neurosurgery and Stanford Stroke Center, Stanford University School of Medicine, Stanford, CA, USA.
Daniel SmerinDepartment of Neurosurgery and Stanford Stroke Center, Stanford University School of Medicine, Stanford, CA, USA.
Lorenzo GonzalezDepartment of Neurosurgery and Stanford Stroke Center, Stanford University School of Medicine, Stanford, CA, USA.
Eric H WangDepartment of Neurosurgery and Stanford Stroke Center, Stanford University School of Medicine, Stanford, CA, USA.
Sabrina LevyDepartment of Neurosurgery and Stanford Stroke Center, Stanford University School of Medicine, Stanford, CA, USA.
Stephanie WangDepartment of Neurosurgery and Stanford Stroke Center, Stanford University School of Medicine, Stanford, CA, USA.
Shunsuke IshizakaDepartment of Neurosurgery and Stanford Stroke Center, Stanford University School of Medicine, Stanford, CA, USA.
Masaki ItoDepartment of Neurosurgery and Stanford Stroke Center, Stanford University School of Medicine, Stanford, CA, USA.
Haruto UchinoDepartment of Neurosurgery and Stanford Stroke Center, Stanford University School of Medicine, Stanford, CA, USA.
Terrance ChiangDepartment of Neurosurgery and Stanford Stroke Center, Stanford University School of Medicine, Stanford, CA, USA.
Michelle Y ChengDepartment of Neurosurgery and Stanford Stroke Center, Stanford University School of Medicine, Stanford, CA, USA. mycheng@stanford.edu.
Gary K SteinbergDepartment of Neurosurgery and Stanford Stroke Center, Stanford University School of Medicine, Stanford, CA, USA. gsteinberg@stanford.edu.
Stanford University · US

Funding

Optogenetic Approaches to Study Post-Stroke Recovery MechanismsR01NS093057 · NINDS · STANFORD UNIVERSITY · PI STEINBERG, GARY K · 2015 to 2025
$5.8M
Optogenetic Approaches to Functional Recovery After StrokeR21NS082894 · NINDS · STANFORD UNIVERSITY · PI STEINBERG, GARY K · 2013 to 2014
$433k
Neurosurgery Research and Education Foundation N/ANIH HHS R25NINDS NIH HHS R01 NS093057NINDS NIH HHS R01NS093057NINDS NIH HHS R21 NS082894NINDS NIH HHS R21NS082894
6 · The paper itself

Abstract

Post-stroke optogenetic stimulation has been shown to enhance neurovascular coupling and functional recovery. Neuronal nitric oxide synthase (nNOS) has been implicated as a key regulator of the neurovascular response in acute stroke; however, its role in subacute recovery remains unclear. We investigated the expression of nNOS in stroke mice undergoing optogenetic stimulation of the contralesional lateral cerebellar nucleus (cLCN). We also examined the effects of nNOS inhibition on functional recovery using a pharmacological inhibitor targeting nNOS. Optogenetically stimulated stroke mice demonstrated significant improvement on the horizontal rotating beam task at post-stroke days 10 and 14. nNOS mRNA and protein expression was significantly and selectively decreased in the contralesional primary motor cortex (cM1) of cLCN-stimulated mice. The nNOS expression in cM1 was negatively correlated with improved recovery. nNOS inhibitor (ARL 17477)-treated stroke mice exhibited a significant functional improvement in speed at post-stroke day 10, when compared to stroke mice receiving vehicle (saline) only. Our results show that optogenetic stimulation of cLCN and systemic nNOS inhibition both produce functional benefits after stroke, and suggest that nNOS may play a maladaptive role in post-stroke recovery.

Indexed as

OptogeneticsStrokeAnimalsMiceNeuronsNitric OxideNitric Oxide Synthase Type IRecovery of FunctionNitric OxideNitric Oxide Synthase Type INos1 protein, mouseBrain stimulationFunctional recoveryNitric oxide synthaseOptogeneticsStroke

Identifiers

PMID32661768
PMCPMC7925487
OpenAlexW3042258933

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.