Evidence map›Paper›PMID 32661657›Full record

ArticleBiotechnology letters2020

Glycerol transporter 1 (Gt1) and zinc-regulated transporter 1 (Zrt1) function in different modes for zinc homeostasis in Komagataella phaffii (Pichia pastoris).

Yankun Yang, Yingyue Pan, Guoqiang Liu, Guibin Dong, Chunjun Zhan, Mattheos A G Koffas, Xiuxia Liu, Ye Li, Chunli Liu, Zhonghu Bai

Abstract read
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Article in Biotechnology letters, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Molecular Response ofJournal of fungi (Basel, Switzerland) · 2023
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Yankun Yang *Key Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China. yangyankun@jiangnan.edu.cn.ORCID http://orcid.org/0000-0003-4815-6832
Yingyue Pan *Key Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Guoqiang LiuKey Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Guibin DongKey Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Chunjun ZhanKey Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Mattheos A G KoffasCenter for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
Xiuxia LiuKey Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Ye LiKey Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Chunli LiuKey Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Zhonghu BaiKey Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Jiangnan University · CNRensselaer Polytechnic Institute · US

Funding

national first-class discipline program of Light Industry Technology and Engineering LITE2018-24National Natural Science Foundation of China 21908077National Natural Science Foundation of China 31570034Six Talent Peaks Project in Jiangsu Province SWYY-180the 111 Project 111-2-06
6 · The paper itself

Abstract

objectivesTo identify the zinc transport function of the membrane proteins Gt1 and Zrt1 in Komagataella phaffii (Pichia pastoris) and study their regulatory mode.

resultsTwo membrane proteins that might have zinc transport function were found in K. phaffii. GT1 was known to encode a glycerol transporter belonging to the Major Facilitator Superfamily. ZRT1 was predicted to resemble the zinc transporter gene in Saccharomyces cerevisiae. Consistent with the prediction, protein plasma-membrane localizations were confirmed by ultracentrifugation and confocal microscopy. Their zinc binding abilities were identified by ITC in vitro, and the impaired zinc uptake activity caused by their deficiencies was confirmed by zinc fluorescence quantification in vivo. Furthermore, zinc excess could turn the two channels off, while zinc deficiency induced their expressions. Gt1 could only function to maintain zinc homeostasis in glycerol, while the block of Gt1 function might lead to Zrt1 upregulation in glucose.

conclusionsThe zinc transport capabilities of Gt1 and Zrt1 were identified in vivo and in vitro. Their regulatory mode to maintain zinc homeostasis in K. phaffii is a new inspiration.

Indexed as

Biological TransportCation Transport ProteinsFungal ProteinsGene Expression Regulation, FungalGlycerolHomeostasisMembrane ProteinsProtein DomainsSaccharomycetalesZincCation Transport ProteinsFungal ProteinsGlycerolMembrane ProteinsZincExpression regulationGlycerol transporter 1Komagataella phaffiiProtein traffickingZinc homeostasisZinc transporter

Identifiers

PMID32661657
OpenAlexW3042529889

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.