Evidence map›Paper›PMID 32661483›Full record

ArticleInternational journal of clinical and experimental pathology2020

Changes in inflammatory factors in SV40MES13 mesangial cells after silencing ApoM gene.

Jiongjiong Liu, Yang Zhang, Xiao Zhang, Shuchang Zhang, Yunfei Hu, Lizhuo Wang, Jialin Gao, Yao Zhang

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in International journal of clinical and experimental pathology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 86% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Jiongjiong LiuDepartment of International Medical Services, The First Affiliated Hospital of USTC-Divsion of Life Sciences and Medicine, University of Sciences and Technology of China Hefei 230001, China.
Yang ZhangAnhui Province Key Laboratory of Biological Macro-Molecules Research, Wannan Medical College Wuhu 241001, China.
Xiao ZhangDepartment of Pediatric Surgery, The First Affiliated Hospital of USTC-Divsion of Life Sciences and Medicine, University of Sciences and Technology of China Hefei 230001, China.
Shuchang ZhangAnhui Province Key Laboratory of Biological Macro-Molecules Research, Wannan Medical College Wuhu 241001, China.
Yunfei HuAnhui Province Key Laboratory of Biological Macro-Molecules Research, Wannan Medical College Wuhu 241001, China.
Lizhuo WangAnhui Province Key Laboratory of Biological Macro-Molecules Research, Wannan Medical College Wuhu 241001, China.
Jialin GaoAnhui Province Key Laboratory of Biological Macro-Molecules Research, Wannan Medical College Wuhu 241001, China.
Yao ZhangAnhui Province Key Laboratory of Biological Macro-Molecules Research, Wannan Medical College Wuhu 241001, China.
Wannan Medical College · CNUniversity of Science and Technology of China · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveInflammation is an important process in the occurrence and development of nephropathy, and ApoM is closely related to inflammation. This article aims to investigate the inflammatory changes of SV40 MES13 cells after ApoM gene silencing by western blot and to explore the relationship between ApoM and inflammation.

methodsControl group glomerular mesangial cells (SV40 MES13), and the same cells after adding a small interfering RNA silencing ApoM gene for 24 h were observed under a microscope and photographed. After extracting the protein western blot was used to explore the associated inflammation of IL-6, P-Jak2, Erk, TNF-α, P-JNK, IKKβ, P-p38, IκBα, P-IKKα/β, NF-κB and P-NF-κB expression.

resultsWestern blot showed that ApoM gene was successfully silenced in SV40 MES13 cells after adding small interfering RNA. The decrease of inflammatory factors IL-6 and P-Jak2 in Jak/Stat pathway was statistically significant. Inflammatory cytokines TNF-α and P-JNK in the NF-κB pathway decreased statistically significantly, while the inflammatory factor IKKβ increased statistically significantly.

conclusionInflammation was suppressed in SV40 cells with ApoM gene silencing.

Indexed as

Apolipoproteininflammationkidney disease

Identifiers

PMID32661483
PMCPMC7344000
OpenAlexW3043181747

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.