ArticleInternational journal of clinical and experimental pathology2020
Hyperplasia suppressor gene inhibits the proliferation and metastasis of glioma cells by targeting rho family proteins.
Article in International journal of clinical and experimental pathology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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8 authors at 4 institutions in 1 country.
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Abstract
aimTo investigate the effect of the hyperplasia suppressor gene (HSG) on human glioma cell invasion and its possible mechanism.
methodsHuman glioma U251 cells were infected with recombinant viral vectors carrying the HSG gene sequence (HSG overexpression group) and HSG interference sequence (HSG suppression group). The negative control group with no-load virus transcription and a blank control group with only PBS treatment were set up. CCK-8 assay, cell scratch healing test, transwell migration, and invasion test were used to detect the effect of HSG expression on proliferation, migration and invasion of U251 glioma cells. Cell immunofluorescence and cell adhesion test were used to analyze the effect of HSG expression on cytoskeleton formation and adhesion ability of U251 cells. Gene chip technology was employed to preliminarily explore the effect of HSG expression change on the inherent gene expression in U251 cells. The expression of Rho family key molecule mRNA and protein was detected by light quantitative PCR and western blot.
resultsAfter 24 h of transcription with the recombinant virus vector, the cells showed a green color under an inverted fluorescence microscope. HSG expression increased in the HSG overexpression group (
conclusionThe overexpression of HSG inhibits the progression of glioma U251 cells by regulating the expression of rho family proteins.
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Identifiers
32661470PMC7344015W3042494182What OpenQuestion holds
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