Evidence map›Paper›PMID 32661427›Full record

ArticleNature methods2020

Novel NanoLuc substrates enable bright two-population bioluminescence imaging in animals.

Yichi Su, Joel R Walker, Yunhee Park, Thomas P Smith, Lan Xiang Liu, Mary P Hall, Louai Labanieh, Robin Hurst, David C Wang, Lance P Encell and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.

0numbers the graph read from it
0cells of the map it votes in
105citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

105 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
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  9. Article
  10. Article
  11. Article
  12. Article
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  14. Article
  15. Article
  16. Article
  17. CaBLAM! A high-contrast bioluminescent CabioRxiv : the preprint server for biology · 2025
    Article
  18. Article
  19. Review
  20. Luciferase complementation for cellular assays beyond protein-protein interactions.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025
    Review

45 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Yichi Su *Department of Neurobiology, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0001-5715-835X
Joel R Walker *Promega Biosciences LLC, San Luis Obispo, CA, USA.
Yunhee ParkDepartment of Neurobiology, Stanford University, Stanford, CA, USA.
Thomas P SmithPromega Biosciences LLC, San Luis Obispo, CA, USA.
Lan Xiang LiuDepartment of Neurobiology, Stanford University, Stanford, CA, USA.
Mary P HallPromega Corporation, Madison, WI, USA.
Louai LabaniehDepartment of Bioengineering, Stanford University, Stanford, CA, USA.
Robin HurstPromega Corporation, Madison, WI, USA.
David C WangDepartment of Neurobiology, Stanford University, Stanford, CA, USA.
Lance P EncellPromega Corporation, Madison, WI, USA.
Namdoo KimDepartment of Neurobiology, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-8584-1823
Feijie ZhangDepartment of Pediatrics, Stanford University, Stanford, CA, USA.
Mark A KayDepartment of Pediatrics, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-2799-2615
Kerriann M CaseyDepartment of Comparative Medicine, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0003-4228-928X
Robbie G MajznerDepartment of Pediatrics, Stanford University, Stanford, CA, USA.
Jennifer R CochranDepartment of Bioengineering, Stanford University, Stanford, CA, USA.
Crystal L MackallDepartment of Pediatrics, Stanford University, Stanford, CA, USA.
Thomas A KirklandPromega Biosciences LLC, San Luis Obispo, CA, USA. thomas.kirkland@promega.com.ORCID http://orcid.org/0000-0003-0859-5785
Michael Z LinDepartment of Neurobiology, Stanford University, Stanford, CA, USA. mzlin@stanford.edu.ORCID http://orcid.org/0000-0002-0492-1961

Funding

Translational Oncology Research Program (Project-005)P30CA124435 · NCI · STANFORD UNIVERSITY · PI Vittorio Sebastiano · 2007 to 2026
$71.4M
Noninvasive bioluminescent imaging of neuronal activity in freely behaving animalsR21DA048252 · NIDA · STANFORD UNIVERSITY · PI LIN, MICHAEL Z. · 2019 to 2020
$435k
NCI NIH HHS P30 CA124435NIDA NIH HHS R21 DA048252
6 · The paper itself

Abstract

Sensitive detection of two biological events in vivo has long been a goal in bioluminescence imaging. Antares, a fusion of the luciferase NanoLuc to the orange fluorescent protein CyOFP, has emerged as a bright bioluminescent reporter with orthogonal substrate specificity to firefly luciferase (FLuc) and its derivatives such as AkaLuc. However, the brightness of Antares in mice is limited by the poor solubility and bioavailability of the NanoLuc substrate furimazine. Here, we report a new substrate, hydrofurimazine, whose enhanced aqueous solubility allows delivery of higher doses to mice. In the liver, Antares with hydrofurimazine exhibited similar brightness to AkaLuc with its substrate AkaLumine. Further chemical exploration generated a second substrate, fluorofurimazine, with even higher brightness in vivo. We used Antares with fluorofurimazine to track tumor size and AkaLuc with AkaLumine to visualize CAR-T cells within the same mice, demonstrating the ability to perform two-population imaging with these two luciferase systems.

Indexed as

AnimalsEnzyme AssaysFuransLuciferasesLuminescent MeasurementsLuminescent ProteinsSubstrate SpecificityFuransLuciferasesLuminescent Proteins

Identifiers

PMID32661427
PMCPMC10907227

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.