ArticleNature chemical biology2020
Acoustic biosensors for ultrasound imaging of enzyme activity.
Article in Nature chemical biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed.
- Real-time volumetric imaging of cells and molecules in deep tissues with Takoyaki ultrasound.Nature communications · 2026Article
- Review
- A programmable genetic platform for engineering noninvasive biosensors.Science advances · 2026Article
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- Temporal gene regulation enables controlled expression of gas vesicles and preserves bacterial viability.Nature communications · 2025Article
- Multiplexed ultrasound imaging of gene expression.Nature methods · 2025Article
- Engineered acoustic reporters for simultaneous tracking of two variables on ultrasonography.Nature methods · 2025Article
- A programmable genetic platform for engineering noninvasive biosensors.bioRxiv : the preprint server for biology · 2025Article
- Nonlinear sound-sheet microscopy: Imaging opaque organs at the capillary and cellular scale.Science (New York, N.Y.) · 2025Article
- Ultrafast optical and passive acoustic mapping characterization of nanoscale cavitation nuclei based on gas vesicle proteins.AIP advances · 2025Article
- Advanced Strategies for Ultrasound Control and Applications in Sonogenetics and Gas Vesicle-Based Technologies: A Review.International journal of nanomedicine · 2025Review
- Harmonic imaging for nonlinear detection of acoustic biomolecules.APL bioengineering · 2024Article
- Elucidating the assembly of gas vesicles by systematic protein-protein interaction analysis.The EMBO journal · 2024Article
- Aptameric Metal-Organic Framework Nanobiosensor.ACS applied nano materials · 2024Article
- Advances in the application of gas vesicles in medical imaging and disease treatment.Journal of biological engineering · 2024Review
- Directed Evolution of Acoustic Reporter Genes Using High-Throughput Acoustic Screening.ACS synthetic biology · 2024Article
- Truly Tiny Acoustic Biomolecules for Ultrasound Imaging and Therapy.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- 50-nm Gas-Filled Protein Nanostructures to Enable the Access of Lymphatic Cells by Ultrasound Technologies.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Next-Generation Genetically Encoded Fluorescent Biosensors Illuminate Cell Signaling and Metabolism.Annual review of biophysics · 2024Review
- Recent advances in ratiometric fluorescence imaging of enzyme activity in vivo.Current opinion in chemical biology · 2024Review
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
Abstract
Visualizing biomolecular and cellular processes inside intact living organisms is a major goal of chemical biology. However, existing molecular biosensors, based primarily on fluorescent emission, have limited utility in this context due to the scattering of light by tissue. In contrast, ultrasound can easily image deep tissue with high spatiotemporal resolution, but lacks the biosensors needed to connect its contrast to the activity of specific biomolecules such as enzymes. To overcome this limitation, we introduce the first genetically encodable acoustic biosensors-molecules that 'light up' in ultrasound imaging in response to protease activity. These biosensors are based on a unique class of air-filled protein nanostructures called gas vesicles, which we engineered to produce nonlinear ultrasound signals in response to the activity of three different protease enzymes. We demonstrate the ability of these biosensors to be imaged in vitro, inside engineered probiotic bacteria, and in vivo in the mouse gastrointestinal tract.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.